EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.91 | - |
Vitis vinifera |
2.4.1.115 | optimized expression in Escherichia coli | Vitis vinifera |
EC Number | General Stability | Organism |
---|---|---|
2.4.1.91 | at least three freeze-thaw cycles can be tolerated without apparent loss of activity | Vitis vinifera |
2.4.1.115 | at least three freeze-thaw cycles can be tolerated without apparent loss of activity | Vitis vinifera |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.91 | Cu2+ | - |
Vitis vinifera | |
2.4.1.91 | Mg2+ | - |
Vitis vinifera | |
2.4.1.91 | Zn2+ | - |
Vitis vinifera | |
2.4.1.115 | Cu2+ | 0.01 mM, over 80% reduction in quercetin glucosylation activity | Vitis vinifera | |
2.4.1.115 | Mn2+ | - |
Vitis vinifera | |
2.4.1.115 | Zn2+ | - |
Vitis vinifera |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.91 | 0.015 | - |
quercetin | recombinant enzyme | Vitis vinifera | |
2.4.1.91 | 0.016 | - |
delphinidin | recombinant enzyme | Vitis vinifera | |
2.4.1.91 | 0.03 | - |
cyanidin | recombinant enzyme | Vitis vinifera | |
2.4.1.91 | 0.0357 | - |
malvidin | recombinant enzyme | Vitis vinifera | |
2.4.1.91 | 1.9 | - |
UDPglucose | recombinant enzyme | Vitis vinifera | |
2.4.1.115 | 0.015 | - |
quercetin | - |
Vitis vinifera | |
2.4.1.115 | 0.016 | - |
delphinidin | - |
Vitis vinifera | |
2.4.1.115 | 0.03 | - |
cyanidin | - |
Vitis vinifera | |
2.4.1.115 | 0.0357 | - |
malvidin | - |
Vitis vinifera | |
2.4.1.115 | 1.9 | - |
UDP-glucose | - |
Vitis vinifera |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.91 | UDPglucose + a flavonol | Vitis vinifera | the principal, if not only, role of the enzyme is to glucosylate anthocyanidins in red fruit during ripening | UDP + a flavonol 3-O-D-glucoside | - |
? | |
2.4.1.115 | additional information | Vitis vinifera | the principal, if not only, role of UDP-glucose:flavonoid 3-O-glucosyltransferase is to glucosylate anthocyanidins in red fruit ripening | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.91 | Vitis vinifera | - |
- |
- |
2.4.1.115 | Vitis vinifera | P51094 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.91 | recombinant enzyme | Vitis vinifera |
2.4.1.115 | - |
Vitis vinifera |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.4.1.91 | fruit | - |
Vitis vinifera | - |
2.4.1.91 | leaf | - |
Vitis vinifera | - |
EC Number | Storage Stability | Organism |
---|---|---|
2.4.1.91 | -20°C or 4°C, the recombinant enzyme is stable for at least 120 h | Vitis vinifera |
2.4.1.91 | -80°C, stable for many months | Vitis vinifera |
2.4.1.115 | -20°C, recombinant enzyme is stable for at least 120 h | Vitis vinifera |
2.4.1.115 | -80°C, recombinant enzyme is stable for many months | Vitis vinifera |
2.4.1.115 | 4°C, recombinant enzyme is stable for at least 120 h | Vitis vinifera |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.91 | additional information | only UDPglucose can serve as glucosyl donor | Vitis vinifera | ? | - |
? | |
2.4.1.91 | UDP-glucose + delphinidin | - |
Vitis vinifera | UDP + delphinidin 3-O-glucoside | - |
? | |
2.4.1.91 | UDPglucose + a flavonol | the principal, if not only, role of the enzyme is to glucosylate anthocyanidins in red fruit during ripening | Vitis vinifera | UDP + a flavonol 3-O-D-glucoside | - |
? | |
2.4.1.91 | UDPglucose + cyanidin | - |
Vitis vinifera | UDP + cyanidin 3-O-glucoside | - |
? | |
2.4.1.91 | UDPglucose + malvidin | - |
Vitis vinifera | UDP + malvidin 3-O-glucoside | - |
? | |
2.4.1.91 | UDPglucose + quercetin | - |
Vitis vinifera | UDP + quercetin 3-O-glucoside | - |
? | |
2.4.1.115 | additional information | the principal, if not only, role of UDP-glucose:flavonoid 3-O-glucosyltransferase is to glucosylate anthocyanidins in red fruit ripening | Vitis vinifera | ? | - |
? | |
2.4.1.115 | additional information | the enzyme is responsible for a late step in anthocyanin biosynthesis | Vitis vinifera | ? | - |
? | |
2.4.1.115 | UDP-alpha-D-glucose + cyanidin | - |
Vitis vinifera | UDP + cyanidin 3-O-beta-D-glucoside | - |
? | |
2.4.1.115 | UDP-alpha-D-glucose + quercetin | no glucosylation with UDP-galactose, UDP-xylose, UDP-glucuronic acid, UDP-mannose, ADP-glucose, CDP-glucose, GDP-glucose or TDP-glucose | Vitis vinifera | UDP + quercetin 3-O-beta-D-glucoside | - |
? | |
2.4.1.115 | UDP-glucose + delphinidin | - |
Vitis vinifera | UDP + delphinidin 3-O-beta-D-glucoside | - |
? | |
2.4.1.115 | UDP-glucose + delphinidin | - |
Vitis vinifera | UDP + delphinidin-3-O-beta-D-glucoside | - |
? | |
2.4.1.115 | UDP-glucose + malvidin | - |
Vitis vinifera | UDP + malvidin 3-O-beta-D-glucoside | - |
? | |
2.4.1.115 | UDP-glucose + malvidin | - |
Vitis vinifera | UDP + malvidin-3-O-beta-D-glucoside | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.115 | UDP-glucose:flavonoid 3-O-glucosyltransferase | - |
Vitis vinifera |
2.4.1.115 | UFGT | - |
Vitis vinifera |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.91 | 8 | - |
reaction with quercetin or cyanidin | Vitis vinifera |
2.4.1.115 | 8 | - |
- |
Vitis vinifera |