Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ishikura, N.; Mato, M.
    Partial purification and some properties of flavonol 3-O-glycosyltransferases from seedlings of Vigna mungo, with special reference to the formation of kaempferol 3-O-galactoside and 3-O-glucoside (1993), Plant Cell Physiol., 34, 329-335.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.91 2-mercaptoethanol 14 mM, stimulates Vigna mungo
2.4.1.91 2-mercaptoethanol 14 mM, activation to 157% Vigna mungo
2.4.1.91 dithioerythritol 10 mM, activation to 215% Vigna mungo
2.4.1.91 EDTA 1 mM, activation to 173% Vigna mungo
2.4.1.234 2-mercaptoethanol 14 mM, activates Vigna mungo
2.4.1.234 dithioerythritol 10 mM, activates Vigna mungo
2.4.1.234 EDTA 1-10 mM, activates Vigna mungo

General Stability

EC Number General Stability Organism
2.4.1.234 14 mM 2-mercaptoethanol stabilizes Vigna mungo

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.91 CaCl2 10 mM, 17% inhibition Vigna mungo
2.4.1.91 CoCl2 1 mM, 35% inhibition Vigna mungo
2.4.1.91 Cu2+ 1 mM CuCl2, 96% inhibition Vigna mungo
2.4.1.91 iodoacetate
-
 Vigna mungo
2.4.1.91 KCl 10 mM, 17% inhibition Vigna mungo
2.4.1.91 Mg2+ 1 mM MgCl2, 18% inhibition Vigna mungo
2.4.1.91 Mn2+ 10 mM MnCl2, 35% inhibition Vigna mungo
2.4.1.91 NEM
-
 Vigna mungo
2.4.1.91 PCMB
-
 Vigna mungo
2.4.1.91 Phenylmercuriacetate
-
 Vigna mungo
2.4.1.91 Zn2+ 1 mM ZnCl2, 96% inhibition Vigna mungo
2.4.1.234 CaCl2 1 mM: 29% inhibition, 10 mM: 53% inhibition Vigna mungo
2.4.1.234 CoCl2 1 mM, 62% inhibition Vigna mungo
2.4.1.234 CuCl2 1 mM, 100% inhibition Vigna mungo
2.4.1.234 iodoacetate 1 mM, 47% inhibition Vigna mungo
2.4.1.234 MgCl2 1 mM: 31% inhibition, 10 mM: 37% inhibition Vigna mungo
2.4.1.234 MnCl2 1 mM: 62% inhibition, 10 mM: 43% inhibition Vigna mungo
2.4.1.234 additional information not inhibited by KCl, iodoacetamide Vigna mungo
2.4.1.234 N-ethylmaleimide 1 mM, 89% inhibition, 14 mM 2-mercaptoethanol completely restores activity Vigna mungo
2.4.1.234 p-chloromercuribenzoate 1 mM, 100% inhibition, 14 mM 2-mercaptoethanol completely restores activity Vigna mungo
2.4.1.234 Phenylmercuriacetate 1 mM, 98% inhibition Vigna mungo
2.4.1.234 ZnCl2 1 mM, 97% inhibition Vigna mungo

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.91 0.00067
-
 kaempferol
-
 Vigna mungo
2.4.1.91 0.69
-
 kaempferol
-
 Vigna mungo
2.4.1.91 1.67
-
 UDP-D-glucose
-
 Vigna mungo
2.4.1.234 0.0004
-
 kaempferol pH 8, 30°C Vigna mungo
2.4.1.234 0.125
-
 UDP-D-galactose pH 8, 30°C, cosubstrate kaempferol Vigna mungo

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.234 soluble
-
 Vigna mungo
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.91 43000
-
 gel filtration Vigna mungo
2.4.1.234 43000
-
 gel filtration Vigna mungo

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.91 Vigna mungo
-
-
-
2.4.1.234 Vigna mungo
-
 NIAS no. R-30
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.91
-
 Vigna mungo
2.4.1.91 partial Vigna mungo
2.4.1.234 404.3fold Vigna mungo

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.234 UDP-alpha-D-galactose + kaempferol = UDP + kaempferol 3-O-beta-D-galactoside acts on the endogenous flavonols kaempferol and quercetin, to a lesser extend on myricetin and fisetin, and weakly on galangin and isorhamnetin, the reaction can occur equally well in both directions Vigna mungo
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.91 seedling
-
 Vigna mungo
-
2.4.1.234 seedling 8 days old Vigna mungo
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.234 0.335
-
 pH 8, 30°C Vigna mungo

Storage Stability

EC Number Storage Stability Organism
2.4.1.91 -20°C, 20 mM imidazole-HCl, pH 8.0, 14 mM 2-mercaptoethanol, 10% glycerol, 35% loss of activity after 2 days, 61% loss of activity after 1 week Vigna mungo
2.4.1.234 -20°C, 20 mM imidazole-HCl, pH 8, 10% glycerol, one week, 100% loss of activity Vigna mungo
2.4.1.234 -20°C, 20 mM imidazole-HCl, pH 8, 14 mM 2-mercaptoethanol, 10% glycerol, 2 days or one week, 35-36% loss of activity Vigna mungo

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.91 UDP-alpha-D-glucose + isorhamnetin
-
 Vigna mungo UDP + isorhamnetin 3-O-beta-D-glucoside
-
 ?
2.4.1.91 UDP-alpha-D-glucose + isorhamnetin 108% of the activity with kaempferol Vigna mungo UDP + isorhamnetin 3-O-beta-D-glucoside
-
 ?
2.4.1.91 UDP-alpha-D-glucose + kaempferol 7-O-glucoside 10% of the activity with kaempferol Vigna mungo UDP + kaempferol 3,7-O-diglucoside
-
 ?
2.4.1.91 UDP-alpha-D-glucose + quercetin 7-O-beta-D-glucoside 12% of the activity with kaempferol Vigna mungo UDP + quercetin 3,7-O-di-beta-D-glucoside
-
 ?
2.4.1.91 UDPglucose + kaempferol
-
 Vigna mungo UDP + kaempferol 3-O-glucoside
-
 ?
2.4.1.91 UDPglucose + kaempferol 5,7,4'-trimethyl ether 9% of the activity with kaempferol Vigna mungo UDP + kaempferol 5,7,4'-trimethyl ether 3-O-glucoside
-
 ?
2.4.1.91 UDPglucose + kaempferol-4'-O-methylether 42% of the activity with kaempferol Vigna mungo UDP + ?
-
 ?
2.4.1.91 UDPglucose + myricetin 20% of the activity with kaempferol Vigna mungo UDP + myrecetin 3-O-glucoside
-
 ?
2.4.1.91 UDPglucose + quercetin
-
 Vigna mungo UDP + quercetin 3-O-glucoside
-
 ?
2.4.1.91 UDPglucose + quercetin 95% of the activity with quercetin Vigna mungo UDP + quercetin 3-O-glucoside
-
 ?
2.4.1.91 UDPglucose + quercetin 5-methyl ether 7% of the activity with kaempferol Vigna mungo UDP + quercetin 5-methyl ether 3-O-glucoside
-
 ?
2.4.1.91 UDPglucose + rhamnetin 98% of the activity with kaempferol Vigna mungo UDP + rhamnetin 3-O-glucoside
-
 ?
2.4.1.234 additional information no/poor substrates: kaempferol 5,7,4’-trimethylether, quercetin 5-monomethylether, kaempferol 3-O-galactoside, kaempferol 7-O-glucoside, kaempferol 7-O-rhamnoside, dihydrokaempferol, dihydroquercetin, eriodictyol Vigna mungo ?
-
 ?
2.4.1.234 UDP-D-galactose + isorhamnetin kaempferol, isorhamnetin and quercetin are the best substrates, F3GaT transfers a galactosyl residue exclusively to the 3-hydroxyl group of the substrate, 106% of the activity with kaempferol as substrate Vigna mungo UDP + isorhamnetin 3-O-beta-D-galactoside
-
 ?
2.4.1.234 UDP-D-galactose + kaempferol kaempferol, isorhamnetin and quercetin are the best substrates, F3GaT transfers a galactosyl residue exclusively to the 3-hydroxyl group of the substrate Vigna mungo UDP + kaempferol 3-O-beta-D-galactoside trifolin ?
2.4.1.234 UDP-D-galactose + kaempferol 4'-monomethylether 79% of the activity with kaempferol as substrate Vigna mungo UDP + kaempferol 4'-monomethylether 3-O-beta-D-galactoside
-
 ?
2.4.1.234 UDP-D-galactose + myricetin 28% of the activity with kaempferol as substrate Vigna mungo UDP + myricetin 3-O-beta-D-galactoside
-
 ?
2.4.1.234 UDP-D-galactose + quercetin kaempferol, isorhamnetin and quercetin are the best substrates, F3GaT transfers a galactosyl residue exclusively to the 3-hydroxyl group of the substrate, 104% of the activity with kaempferol as substrate Vigna mungo UDP + quercetin 3-O-beta-D-galactoside
-
 ?
2.4.1.234 UDP-D-galactose + quercetin 7-O-glucoside 33% of the activity with kaempferol as substrate Vigna mungo UDP + quercetin 7-O-glucoside-3-O-beta-D-galactoside
-
 ?
2.4.1.234 UDP-D-galactose + rhamnetin 75% of the activity with kaempferol as substrate Vigna mungo UDP + rhamnetin 3-O-beta-D-galactoside
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.234 F3GalTase
-
 Vigna mungo
2.4.1.234 F3GaT
-
 Vigna mungo
2.4.1.234 flavonoid 3-O-galactosyltransferase
-
 Vigna mungo
2.4.1.234 flavonol 3-O-galactosyltransferase
-
 Vigna mungo
2.4.1.234 galactosyltransferase, uridine diphosphogalactose-flavonol 3-O-
-
 Vigna mungo
2.4.1.234 UDP-galactose:flavonoid 3-O-galactosyltransferase
-
 Vigna mungo
2.4.1.234 UDP-galactose:flavonol 3-O-galactosyltransferase
-
 Vigna mungo
2.4.1.234 UDPgalactose-flavonoid-3-O-glycosyltransferase
-
 Vigna mungo
2.4.1.234 uridine diphosphogalactose-flavonol 3-O-galactosyltransferase
-
 Vigna mungo

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.91 7.5
-
 in histidine-HCl buffer Vigna mungo
2.4.1.91 8
-
 in imidazole-HCl buffer Vigna mungo
2.4.1.234 7.5
-
 in histidine-HCl buffer Vigna mungo
2.4.1.234 8
-
 in imidazole-HCl buffer Vigna mungo

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.1.234 Vigna mungo chromatofocusing
-
 6