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Literature summary extracted from

  • Malissard, M.; Borsig, L.; Di Marco, S.; Gruetter, M.G.; Kragl, U.; Wandrey, C.; Berger, E.G.
    Recombinant soluble beta-1,4-galactosyltransferases expressed in Saccharomyces cerevisiae. Purification, characterization and comparison with human enzyme (1996), Eur. J. Biochem., 239, 340-348.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.38 alpha-lactalbumin stimulates transfer of galactose from UDPgalactose to N-acetylgalactosamine Homo sapiens
2.4.1.90 alpha-lactalbumin stimulates transfer of galactose from UDPgalactose to N-acetylgalactosamine Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.38 enzymatically active soluble N-deglycosylated enzyme form Homo sapiens
2.4.1.90 enzymatically active soluble N-deglycosylated enzyme form Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.38 additional information
-
 additional information Km-value for ovalbumin for the N-deglycosylated recombinant enzyme is 6 mg/ml, and 15 mg/ml for th recombinant enzyme Homo sapiens
2.4.1.38 0.091
-
 UDPgalactose recombinant enzyme Homo sapiens
2.4.1.38 0.106
-
 UDPgalactose
-
 Homo sapiens
2.4.1.38 0.143
-
 UDPgalactose recombinant N-deglycosylated enzyme Homo sapiens
2.4.1.38 0.2
-
 UDPgalactose reaction with glucose, wild-type enzyme and N-deglycosylated recombinant enzyme Homo sapiens
2.4.1.38 0.21
-
 UDPgalactose reaction with glucose, recombinant enzyme Homo sapiens
2.4.1.38 1
-
 D-glucose recombinant enzyme Homo sapiens
2.4.1.38 1.1
-
 D-glucose recombinant N-deglycosylated enzyme Homo sapiens
2.4.1.38 2
-
 D-glucose
-
 Homo sapiens
2.4.1.38 3.3
-
 N-acetylglucosamine
-
 Homo sapiens
2.4.1.38 8.3
-
 N-acetylglucosamine recombinant N-deglycosylated enzyme Homo sapiens
2.4.1.38 10
-
 N-acetylglucosamine recombinant enzyme Homo sapiens
2.4.1.90 additional information
-
 additional information Km-value for ovalbumin for the N-deglycosylated recombinant enzyme is 6 mg/ml, and 15 mg/ml for th recombinant enzyme Homo sapiens
2.4.1.90 0.091
-
 UDPgalactose recombinant enzyme Homo sapiens
2.4.1.90 0.106
-
 UDPgalactose
-
 Homo sapiens
2.4.1.90 0.143
-
 UDPgalactose recombinant N-deglycosylated enzyme Homo sapiens
2.4.1.90 0.2
-
 UDPgalactose reaction with glucose, wild-type enzyme and N-deglycosylated recombinant enzyme Homo sapiens
2.4.1.90 0.21
-
 UDPgalactose reaction with glucose, recombinant enzyme Homo sapiens
2.4.1.90 1
-
 D-glucose recombinant enzyme Homo sapiens
2.4.1.90 1.1
-
 D-glucose recombinant N-deglycosylated enzyme Homo sapiens
2.4.1.90 2
-
 D-glucose
-
 Homo sapiens
2.4.1.90 3.3
-
 N-acetylglucosamine
-
 Homo sapiens
2.4.1.90 8.3
-
 N-acetylglucosamine recombinant N-deglycosylated enzyme Homo sapiens
2.4.1.90 10
-
 N-acetylglucosamine recombinant enzyme Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.38 55000
-
 2 * 55000, SDS-PAGE Homo sapiens
2.4.1.38 106000
-
 calculation from light-scattering experiments Homo sapiens
2.4.1.90 55000
-
 2 * 55000, SDS-PAGE Homo sapiens
2.4.1.90 106000
-
 calculation from light-scattering experiments Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.38 Homo sapiens
-
-
-
2.4.1.90 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.38 glycoprotein
-
 Homo sapiens
2.4.1.90 glycoprotein
-
 Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.38 recombinant enzyme Homo sapiens
2.4.1.90 recombinant enzyme Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.38 milk
-
 Homo sapiens
-
2.4.1.90 milk
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.38 6.9
-
 N-deglycosylated recombinant enzyme Homo sapiens
2.4.1.38 8.4
-
 recombinant enzyme Homo sapiens
2.4.1.90 6.9
-
 N-deglycosylated recombinant enzyme Homo sapiens
2.4.1.90 8.4
-
 recombinant enzyme Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.38 UDPgalactose + glucose in presence of alpha-lactalbumin Homo sapiens lactose + UDP
-
 ?
2.4.1.38 UDPgalactose + N-acetylglucosamine
-
 Homo sapiens UDP + N-acetyllactosamine
-
 ?
2.4.1.90 UDPgalactose + glucose in presence of alpha-lactalbumin Homo sapiens lactose + UDP
-
 ?
2.4.1.90 UDPgalactose + N-acetylglucosamine
-
 Homo sapiens UDP + N-acetyllactosamine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.38 dimer 2 * 55000, SDS-PAGE Homo sapiens
2.4.1.90 dimer 2 * 55000, SDS-PAGE Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.38 40
-
 4 h, 18% loss of activity Homo sapiens
2.4.1.38 50
-
 1 h, complete loss of activity Homo sapiens
2.4.1.90 40
-
 4 h, 18% loss of activity Homo sapiens
2.4.1.90 50
-
 1 h, complete loss of activity Homo sapiens