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Literature summary extracted from

  • Ramakrishnan, B.; Qasba, P.K.
    Structure-based design of beta 1,4-galactosyltransferase I (beta4Gal-T1) with equally efficient N-acetylgalactosaminyltransferase activity: point mutation broadens beta 4Gal-T1 donor specificity (2002), J. Biol. Chem., 277, 20833-20839.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.38 alpha-lactalbumin stimulates transfer of galactose from UDPgalactose to N-acetylgalactosamine Bos taurus
2.4.1.90 alpha-lactalbumin stimulates transfer of galactose from UDPgalactose to N-acetylgalactosamine Bos taurus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.1.38
-
 Bos taurus
2.4.1.90
-
 Bos taurus

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.38 Y289I mutation enhances GalNAc-transferase activity. Km for GlcNAc is increased compared to the wild type Bos taurus
2.4.1.38 Y289L mutation enhances GalNAc-transferase activity. Km for GlcNAc is incereased compared to the wild type Bos taurus
2.4.1.38 Y289N mutation enhances GalNAc-transferase activity. Km for GlcNAc is increased compared to the wild type Bos taurus
2.4.1.90 Y289I mutation enhances GalNAc-transferase activity. Km for GlcNAc is increased compared to the wild type Bos taurus
2.4.1.90 Y289L mutation enhances GalNAc-transferase activity. Km for GlcNAc is incereased compared to the wild type Bos taurus
2.4.1.90 Y289N mutation enhances GalNAc-transferase activity. Km for GlcNAc is increased compared to the wild type Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.38 Mn2+ required Bos taurus
2.4.1.90 Mn2+ required Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.38 Bos taurus
-
-
-
2.4.1.90 Bos taurus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.38 UDP-GalNAc + GlcNAc transfer of GalNAc is only 1% of galactose transfer in wild type enzyme. Mutant enzyme Y289L exhibits nearly 100% of the galactose transferase activity Bos taurus ?
-
 ?
2.4.1.38 UDPgalactose + glucose in presence of alpha-lactalbumin Bos taurus lactose + UDP
-
 ?
2.4.1.38 UDPgalactose + N-acetylglucosamine
-
 Bos taurus UDP + N-acetyllactosamine
-
 ?
2.4.1.90 UDP-GalNAc + GlcNAc transfer of GalNAc is only 1% of galactose transfer in wild type enzyme. Mutant enzyme Y289L exhibits nearly 100% of the galactose transferase activity Bos taurus ?
-
 ?
2.4.1.90 UDPgalactose + glucose in presence of alpha-lactalbumin Bos taurus lactose + UDP
-
 ?
2.4.1.90 UDPgalactose + N-acetylglucosamine
-
 Bos taurus UDP + N-acetyllactosamine
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.38 additional information
-
 additional information
-
 Bos taurus
2.4.1.90 additional information
-
 additional information
-
 Bos taurus