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Literature summary extracted from

  • Nakazawa, K.; Furukawa, K.; Narimatsu, H.; Kobata, A.
    Kinetic study of human beta-1,4-galactosyltransferase expressed in E. coli (1993), J. Biochem., 113, 747-753.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.38 expression in Escherichia coli Homo sapiens
2.4.1.90 expression in Escherichia coli Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.38 0.025
-
 UDPgalactose recombinant enzyme Homo sapiens
2.4.1.38 0.064
-
 asialo-agalacto-transferrin recombinant enzyme Homo sapiens
2.4.1.38 0.17
-
 agalacto-poly-N-acetyllactosamine
-
 Homo sapiens
2.4.1.38 0.19
-
 lacto-N-triose II
-
 Homo sapiens
2.4.1.38 0.83
-
 lacto-N-triaosylceramide
-
 Homo sapiens
2.4.1.38 2.5
-
 N-acetylglucosamine recombinant enzyme Homo sapiens
2.4.1.90 0.025
-
 UDPgalactose recombinant enzyme Homo sapiens
2.4.1.90 0.064
-
 asialo-agalacto-transferrin recombinant enzyme Homo sapiens
2.4.1.90 0.17
-
 agalacto-poly-N-acetyllactosamine
-
 Homo sapiens
2.4.1.90 0.19
-
 lacto-N-triose II
-
 Homo sapiens
2.4.1.90 0.83
-
 lacto-N-triaosylceramide
-
 Homo sapiens
2.4.1.90 2.5
-
 N-acetylglucosamine recombinant enzyme Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.38 38000
-
 x * 38000, SDS-PAGE Homo sapiens
2.4.1.90 38000
-
 x * 38000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.38 UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides Homo sapiens the enzyme may be involved in the synthesis of poly-N-acetyllactosamine, lacto-N-neotetraose and probably lacto-N-neotetraosylceramide in addition to the formation of the Galbeta1-4GlcNAc group of glycoprotein sugar chains and lactose ?
-
 ?
2.4.1.90 UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides Homo sapiens the enzyme may be involved in the synthesis of poly-N-acetyllactosamine, lacto-N-neotetraose and probably lacto-N-neotetraosylceramide in addition to the formation of the Galbeta1-4GlcNAc group of glycoprotein sugar chains and lactose ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.38 Homo sapiens
-
-
-
2.4.1.90 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.38 recombinant enzyme Homo sapiens
2.4.1.90 recombinant enzyme Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.38 UDP-alpha-D-galactose + asialo-agalacto-transferrin
-
 Homo sapiens ?
-
 ?
2.4.1.38 UDPgalactose + agalacto-poly-N-acetyllactosamine
-
 Homo sapiens ?
-
 ?
2.4.1.38 UDPgalactose + lacto-N-triaosylceramide
-
 Homo sapiens ?
-
 ?
2.4.1.38 UDPgalactose + lacto-N-triose II
-
 Homo sapiens ?
-
 ?
2.4.1.38 UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides the enzyme may be involved in the synthesis of poly-N-acetyllactosamine, lacto-N-neotetraose and probably lacto-N-neotetraosylceramide in addition to the formation of the Galbeta1-4GlcNAc group of glycoprotein sugar chains and lactose Homo sapiens ?
-
 ?
2.4.1.90 UDP-alpha-D-galactose + asialo-agalacto-transferrin
-
 Homo sapiens ?
-
 ?
2.4.1.90 UDPgalactose + agalacto-poly-N-acetyllactosamine
-
 Homo sapiens ?
-
 ?
2.4.1.90 UDPgalactose + lacto-N-triaosylceramide
-
 Homo sapiens ?
-
 ?
2.4.1.90 UDPgalactose + lacto-N-triose II
-
 Homo sapiens ?
-
 ?
2.4.1.90 UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides the enzyme may be involved in the synthesis of poly-N-acetyllactosamine, lacto-N-neotetraose and probably lacto-N-neotetraosylceramide in addition to the formation of the Galbeta1-4GlcNAc group of glycoprotein sugar chains and lactose Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.38 ? x * 38000, SDS-PAGE Homo sapiens
2.4.1.90 ? x * 38000, SDS-PAGE Homo sapiens