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Literature summary extracted from

  • Rao, A.K.; Garver, F.; Mendicino, J.
    Biosynthesis of the carbohydrate units of immunoglobulins. 1. Purification and properties of galactosyltransferases from swine mesentary lymph nodes (1976), Biochemistry, 15, 5001-5009.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.38 N-acetylglucosamine competitively inhibits the transfer of galactose to glycoprotein substrates Sus scrofa
2.4.1.38 p-nitrophenyl 2-acetamido-2-deoxy-beta-glucoside competitively inhibits the transfer of galactose to glycoprotein substrates Sus scrofa
2.4.1.38 UDP competitively inhibits the transfer of galactose to glycoprotein substrates Sus scrofa
2.4.1.38 UMP competitively inhibits the transfer of galactose to glycoprotein substrates Sus scrofa
2.4.1.90 N-acetylglucosamine competitively inhibits the transfer of galactose to glycoprotein substrates Sus scrofa
2.4.1.90 p-nitrophenyl 2-acetamido-2-deoxy-beta-glucoside competitively inhibits the transfer of galactose to glycoprotein substrates Sus scrofa
2.4.1.90 UDP competitively inhibits the transfer of galactose to glycoprotein substrates Sus scrofa
2.4.1.90 UMP competitively inhibits the transfer of galactose to glycoprotein substrates Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.38 0.02
-
 immunoglobulin G heavy chain
-
 Sus scrofa
2.4.1.38 0.022
-
 UDPgalactose
-
 Sus scrofa
2.4.1.38 0.029
-
 fetuin
-
 Sus scrofa
2.4.1.38 0.054
-
 ovalbumin
-
 Sus scrofa
2.4.1.38 0.25
-
 glycopeptide from porcine immunoglobulin G
-
 Sus scrofa
2.4.1.38 0.66
-
 p-nitrophenyl 2-acetamido-2-deoxy-beta-glucoside
-
 Sus scrofa
2.4.1.38 5.8
-
 N-acetylglucosamine
-
 Sus scrofa
2.4.1.90 0.02
-
 immunoglobulin G heavy chain
-
 Sus scrofa
2.4.1.90 0.022
-
 UDPgalactose
-
 Sus scrofa
2.4.1.90 0.029
-
 fetuin
-
 Sus scrofa
2.4.1.90 0.054
-
 ovalbumin
-
 Sus scrofa
2.4.1.90 0.25
-
 glycopeptide from porcine immunoglobulin G
-
 Sus scrofa
2.4.1.90 0.66
-
 p-nitrophenyl 2-acetamido-2-deoxy-beta-glucoside
-
 Sus scrofa
2.4.1.90 5.8
-
 N-acetylglucosamine
-
 Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.38 membrane
-
 Sus scrofa 16020
-
2.4.1.38 soluble
-
 Sus scrofa
-
-
2.4.1.90 membrane
-
 Sus scrofa 16020
-
2.4.1.90 soluble
-
 Sus scrofa
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.38 57000
-
 sucrose density gradient centrifugation Sus scrofa
2.4.1.90 57000
-
 sucrose density gradient centrifugation Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.38 Sus scrofa
-
-
-
2.4.1.90 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.38
-
 Sus scrofa
2.4.1.90
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.38 mesenteric lymph node
-
 Sus scrofa
-
2.4.1.90 mesenteric lymph node
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.38 0.035
-
-
 Sus scrofa
2.4.1.90 0.035
-
-
 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
2.4.1.38 -20°C, 0.02 M Tris/HCl buffer, pH 7.5, stable for several months Sus scrofa
2.4.1.90 -20°C, 0.02 M Tris/HCl buffer, pH 7.5, stable for several months Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.38 UDP-alpha-D-galactose + glycopeptide from porcine immunoglobulin G
-
 Sus scrofa ?
-
 ?
2.4.1.38 UDP-alpha-D-galactose + immunoglobulin G heavy chain
-
 Sus scrofa ?
-
 ?
2.4.1.38 UDPgalactose + fetuin
-
 Sus scrofa ?
-
 ?
2.4.1.38 UDPgalactose + immunoglobulin heavy chain
-
 Sus scrofa ?
-
 ?
2.4.1.38 UDPgalactose + N-acetyl-beta-D-glucosaminyl-glycopeptide glycopeptide prepared from porcine IgG immunoglobulin Sus scrofa UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminylglycopeptide
-
 ?
2.4.1.38 UDPgalactose + N-acetylglucosamine
-
 Sus scrofa UDP + N-acetyllactosamine
-
 ?
2.4.1.38 UDPgalactose + ovalbumin
-
 Sus scrofa ?
-
 ?
2.4.1.38 UDPgalactose + p-nitrophenyl 2-acetamido-2-deoxy-beta-glucoside
-
 Sus scrofa ?
-
 ?
2.4.1.90 UDP-alpha-D-galactose + glycopeptide from porcine immunoglobulin G
-
 Sus scrofa ?
-
 ?
2.4.1.90 UDP-alpha-D-galactose + immunoglobulin G heavy chain
-
 Sus scrofa ?
-
 ?
2.4.1.90 UDPgalactose + fetuin
-
 Sus scrofa ?
-
 ?
2.4.1.90 UDPgalactose + immunoglobulin heavy chain
-
 Sus scrofa ?
-
 ?
2.4.1.90 UDPgalactose + N-acetyl-beta-D-glucosaminyl-glycopeptide glycopeptide prepared from porcine IgG immunoglobulin Sus scrofa UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminylglycopeptide
-
 ?
2.4.1.90 UDPgalactose + N-acetylglucosamine
-
 Sus scrofa UDP + N-acetyllactosamine
-
 ?
2.4.1.90 UDPgalactose + ovalbumin
-
 Sus scrofa ?
-
 ?
2.4.1.90 UDPgalactose + p-nitrophenyl 2-acetamido-2-deoxy-beta-glucoside
-
 Sus scrofa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.38 monomer 1 * 57000, SDS-PAGE Sus scrofa
2.4.1.90 monomer 1 * 57000, SDS-PAGE Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.38 6.8
-
-
 Sus scrofa
2.4.1.90 6.8
-
-
 Sus scrofa