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Literature summary extracted from

  • Bouchilloux, S.
    Purification by affinity chromatography and some properties of microsomal galactosyltransferase from pig thyroid (1979), Biochim. Biophys. Acta, 569, 135-144.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.38 EDTA
-
 Sus scrofa
2.4.1.90 EDTA
-
 Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.38 0.08
-
 UDPgalactose
-
 Sus scrofa
2.4.1.38 4
-
 N-acetylglucosamine
-
 Sus scrofa
2.4.1.90 0.08
-
 UDPgalactose
-
 Sus scrofa
2.4.1.90 4
-
 N-acetylglucosamine
-
 Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.38 Golgi apparatus
-
 Sus scrofa 5794
-
2.4.1.38 membrane
-
 Sus scrofa 16020
-
2.4.1.38 microsome
-
 Sus scrofa
-
-
2.4.1.90 Golgi apparatus
-
 Sus scrofa 5794
-
2.4.1.90 membrane
-
 Sus scrofa 16020
-
2.4.1.90 microsome
-
 Sus scrofa
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.38 Mn2+ required Sus scrofa
2.4.1.38 Mn2+ optimal concentration is 5-10 mM MnCl2 Sus scrofa
2.4.1.90 Mn2+ required Sus scrofa
2.4.1.90 Mn2+ optimal concentration is 5-10 mM MnCl2 Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.38 70000
-
 gel filtration Sus scrofa
2.4.1.38 74000
-
 1 * 74000, SDS-PAGE Sus scrofa
2.4.1.90 70000
-
 gel filtration Sus scrofa
2.4.1.90 74000
-
 1 * 74000, SDS-PAGE Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.38 Sus scrofa
-
-
-
2.4.1.90 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.38
-
 Sus scrofa
2.4.1.90
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.38 thyroid gland
-
 Sus scrofa
-
2.4.1.90 thyroid gland
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.38 additional information
-
-
 Sus scrofa
2.4.1.90 additional information
-
-
 Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.38 UDPgalactose + degalactosylated fetuin
-
 Sus scrofa UDP + fetuin containing beta-1,4-galactose linkages
-
 ?
2.4.1.38 UDPgalactose + N-acetylglucosamine
-
 Sus scrofa UDP + N-acetyllactosamine
-
 ?
2.4.1.90 UDPgalactose + degalactosylated fetuin
-
 Sus scrofa UDP + fetuin containing beta-1,4-galactose linkages
-
 ?
2.4.1.90 UDPgalactose + N-acetylglucosamine
-
 Sus scrofa UDP + N-acetyllactosamine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.38 monomer 1 * 74000, SDS-PAGE Sus scrofa
2.4.1.90 monomer 1 * 74000, SDS-PAGE Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.38 37
-
 activity at 37°C is faster than at 31°C or at 27°C Sus scrofa
2.4.1.90 37
-
 activity at 37°C is faster than at 31°C or at 27°C Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.38 6.4 7.6
-
 Sus scrofa
2.4.1.90 6.4 7.6
-
 Sus scrofa