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Literature summary extracted from

  • Nakazawa, K.; Furukawa, K.; Kobata, A.; Narimatsu, H.
    Characterization of a murine beta 1-4 galactosyltransferase expressed in COS-1 cells (1991), Eur. J. Biochem., 196, 363-368.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.38 alpha-lactalbumin stimulates transfer of galactose from UDPgalactose to N-acetylgalactosamine Mus musculus
2.4.1.90 alpha-lactalbumin stimulates transfer of galactose from UDPgalactose to N-acetylgalactosamine Mus musculus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.38 expression in Cos-1 cells Mus musculus
2.4.1.90 expression in Cos-1 cells Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.38 alpha-lactalbumin partially inhibits reaction with UDPgalactose and asialo-agalacto-transferrin Mus musculus
2.4.1.38 EDTA
-
 Mus musculus
2.4.1.90 alpha-lactalbumin partially inhibits reaction with UDPgalactose and asialo-agalacto-transferrin Mus musculus
2.4.1.90 EDTA
-
 Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.38 0.024
-
 UDPgalactose enzyme in pCMGT1-transfected cells Mus musculus
2.4.1.38 0.056
-
 asialo-agalacto-transferrin enzyme in pCMGT1-transfected cells Mus musculus
2.4.1.38 1.9
-
 N-acetylglucosamine enzyme in pCMGT1-transfected cells Mus musculus
2.4.1.90 0.024
-
 UDPgalactose enzyme in pCMGT1-transfected cells Mus musculus
2.4.1.90 0.056
-
 asialo-agalacto-transferrin enzyme in pCMGT1-transfected cells Mus musculus
2.4.1.90 1.9
-
 N-acetylglucosamine enzyme in pCMGT1-transfected cells Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.38 Mn2+ required Mus musculus
2.4.1.38 Mn2+ optimal concentration is 10 mM Mus musculus
2.4.1.90 Mn2+ required Mus musculus
2.4.1.90 Mn2+ optimal concentration is 10 mM Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.38 Mus musculus
-
-
-
2.4.1.90 Mus musculus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.38 UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide the sequences of cDNA isolated from mammary and F9 cell lines are identical, thus indicating that EC 2.4.1.38 and EC 2.4.1.90 are non-distinguishable Mus musculus
a
2.4.1.90 UDP-alpha-D-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine the sequences of cDNA isolated from mammary and F9 cell lines are identical, thus indicating that EC 2.4.1.38 and EC 2.4.1.90 are non-distinguishable Mus musculus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.38 F9 cell
-
 Mus musculus
-
2.4.1.90 F9 cell
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.38 UDPgalactose + asialo-agalacto-transferrin transfer of galactose to N-acetylglucosamine residues of Asn-linked sugar chains of glycoproteins in a beta1-4linkage Mus musculus ?
-
 ?
2.4.1.38 UDPgalactose + glucose in presence of alpha-lactalbumin Mus musculus lactose + UDP
-
 ?
2.4.1.38 UDPgalactose + N-acetylglucosamine
-
 Mus musculus UDP + N-acetyllactosamine
-
 ?
2.4.1.90 UDPgalactose + asialo-agalacto-transferrin transfer of galactose to N-acetylglucosamine residues of Asn-linked sugar chains of glycoproteins in a beta1-4linkage Mus musculus ?
-
 ?
2.4.1.90 UDPgalactose + glucose in presence of alpha-lactalbumin Mus musculus lactose + UDP
-
 ?
2.4.1.90 UDPgalactose + N-acetylglucosamine
-
 Mus musculus UDP + N-acetyllactosamine
-
 ?