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Literature summary extracted from

  • Helting, T.; Erbing, B.
    Galactosyltransfer in mouse mastocytoma: purification and properties of N-acetyllactosamine synthetase (1973), Biochim. Biophys. Acta, 293, 94-104.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.38 alpha-lactalbumin stimulates transfer of galactose from UDPgalactose to N-acetylgalactosamine Mus musculus
2.4.1.90 alpha-lactalbumin stimulates transfer of galactose from UDPgalactose to N-acetylgalactosamine Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.38 0.25
-
 UDPgalactose
-
 Mus musculus
2.4.1.38 1
-
 N-acetylglucosamine
-
 Mus musculus
2.4.1.90 0.25
-
 UDPgalactose
-
 Mus musculus
2.4.1.90 1
-
 N-acetylglucosamine
-
 Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.38 microsome
-
 Mus musculus
-
-
2.4.1.90 microsome
-
 Mus musculus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.38 Ca2+ can partially replace Mn2+ Mus musculus
2.4.1.38 Mg2+ can partially replace Mn2+ Mus musculus
2.4.1.38 Mn2+ required Mus musculus
2.4.1.90 Ca2+ can partially replace Mn2+ Mus musculus
2.4.1.90 Mg2+ can partially replace Mn2+ Mus musculus
2.4.1.90 Mn2+ required Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.38 Mus musculus
-
-
-
2.4.1.90 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.38
-
 Mus musculus
2.4.1.90
-
 Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.38 mastocytoma cell
-
 Mus musculus
-
2.4.1.90 mastocytoma cell
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.38 UDPgalactose + glucose in presence of alpha-lactalbumin Mus musculus lactose + UDP
-
 ?
2.4.1.38 UDPgalactose + N-acetylglucosamine
-
 Mus musculus UDP + N-acetyllactosamine
-
 ?
2.4.1.90 UDPgalactose + glucose in presence of alpha-lactalbumin Mus musculus lactose + UDP
-
 ?
2.4.1.90 UDPgalactose + N-acetylglucosamine
-
 Mus musculus UDP + N-acetyllactosamine
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.38 8.2
-
-
 Mus musculus
2.4.1.90 8.2
-
-
 Mus musculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.38 5 9.3 pH 5.0: about 60% of maximal activity, pH 9.3: about 45% of maximal activity Mus musculus
2.4.1.90 5 9.3 pH 5.0: about 60% of maximal activity, pH 9.3: about 45% of maximal activity Mus musculus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.38 5 9
-
 Mus musculus
2.4.1.90 5 9
-
 Mus musculus