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Literature summary extracted from

  • Park, J.P.; Bae, J.T.; Yun, J.W.
    Critical effect of ammonium ions on the enzymatic reaction of a novel transfructosylating enzyme for fructooligosaccharide production from sucrose (1999), Biotechnol. Lett., 21, 987-990.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.9 (NH4)NO3 10 mM, 15fold enhancement of activity Paenibacillus macerans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.9 Fe2+ slightly enhances activity Paenibacillus macerans
2.4.1.9 K+ slightly enhances activity Paenibacillus macerans
2.4.1.9 Mg2+ slightly enhances activity Paenibacillus macerans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.9 sucrose + [(beta-D-Fruf-(2-1))n]-alpha-D-Glup Paenibacillus macerans
-
 alpha-D-glucose + [(beta-D-Fruf-(2-1))n+1]-alpha-D-Glup
-
 ?
2.4.1.9 sucrose + [(beta-D-Fruf-(2-1))n]-alpha-D-Glup Paenibacillus macerans EG-7
-
 alpha-D-glucose + [(beta-D-Fruf-(2-1))n+1]-alpha-D-Glup
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.9 Paenibacillus macerans
-
 EG-7
-
2.4.1.9 Paenibacillus macerans EG-7
-
 EG-7
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.9 partial Paenibacillus macerans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.9 sucrose + sucrose
-
 Paenibacillus macerans [(beta-D-Fruf-(2-1))5]-alpha-D-Glup + alpha-D-glucose the enzyme selectively produces GF5 from sucose ?
2.4.1.9 sucrose + sucrose
-
 Paenibacillus macerans EG-7 [(beta-D-Fruf-(2-1))5]-alpha-D-Glup + alpha-D-glucose the enzyme selectively produces GF5 from sucose ?
2.4.1.9 sucrose + [(beta-D-Fruf-(2-1))n]-alpha-D-Glup
-
 Paenibacillus macerans alpha-D-glucose + [(beta-D-Fruf-(2-1))n+1]-alpha-D-Glup
-
 ?
2.4.1.9 sucrose + [(beta-D-Fruf-(2-1))n]-alpha-D-Glup the enzyme selectively produces GF5 from sucose Paenibacillus macerans alpha-D-glucose + [(beta-D-Fruf-(2-1))n+1]-alpha-D-Glup
-
 ?
2.4.1.9 sucrose + [(beta-D-Fruf-(2-1))n]-alpha-D-Glup
-
 Paenibacillus macerans EG-7 alpha-D-glucose + [(beta-D-Fruf-(2-1))n+1]-alpha-D-Glup
-
 ?
2.4.1.9 sucrose + [(beta-D-Fruf-(2-1))n]-alpha-D-Glup the enzyme selectively produces GF5 from sucose Paenibacillus macerans EG-7 alpha-D-glucose + [(beta-D-Fruf-(2-1))n+1]-alpha-D-Glup
-
 ?