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Literature summary extracted from

  • Xu, H.; Storch, T.; Yu, M.; Elliott, S.P.; Haslam, D.B.
    Characterization of the human Forssman synthetase gene. An evolving association between glycolipid synthesis and host-microbial interactions (1999), J. Biol. Chem., 274, 29390-29398.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.88 cloned from cDNA library by PCR, expression, transcription and processing, of wild-type in COS-1 cells with and without HA-tag, but no detectable enzyme activity in the transfected cells by recombinant enzyme, DNA and amino acid sequence determination and analysis Homo sapiens
2.4.1.88 expression in COS-1 cells with and without HA-tag Canis lupus familiaris
2.4.1.88 expression of wild-type in T42 human bladder and Vero monkey kidney epithelial cells, enables the cells to bind a canine Escherichia coli uropathogen, which they cannot bind without transfection Canis lupus familiaris

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.88 additional information chimeric enzyme constructs of N-terminal canine sequence fused in frame with the C-terminal catalytic region of the human sequence, i.e. dog:human construct, and vice versa, i.e. human:dog construct, expression in COS-1 cells, activity is shown only by the human:dog construct Canis lupus familiaris
2.4.1.88 additional information chimeric enzyme constructs of N-terminal canine sequence fused in frame with the C-terminal catalytic region of the human sequence, i.e. dog:human construct, and vice versa, i.e. human:dog construct, expression in COS-1 cells, activity is shown only by the human:dog construct Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.88 membrane
-
 Canis lupus familiaris 16020
-
2.4.1.88 membrane
-
 Homo sapiens 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.88 additional information
-
 amino acid sequence alignment and comparison, 83% identity Canis lupus familiaris
2.4.1.88 additional information
-
 amino acid sequence alignment and comparison, 83% identity Homo sapiens
2.4.1.88 additional information
-
 genomic organisation Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.88 additional information Homo sapiens variability in glycolipid synthesis between species is an important determinant of microbial tropism ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.88 Canis lupus familiaris
-
-
-
2.4.1.88 Homo sapiens
-
 cosmid sequence from chromosome 9q34
-
2.4.1.88 Homo sapiens Q8N5D6 immunoreactive sequence homologue to FS sequences, but without FS activity due to modification of catalytic domain during evolution
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.88 additional information enzyme mRNA is detectable in all tissues Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.88 additional information variability in glycolipid synthesis between species is an important determinant of microbial tropism Homo sapiens ?
-
 ?
2.4.1.88 UDP-N-acetyl-D-galactosamine + N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide no activity Homo sapiens UDP + N-acetyl-D-galactosaminyl-1,3-N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.88 Forssmann glycolipid synthetase
-
 Canis lupus familiaris
2.4.1.88 Forssmann glycolipid synthetase
-
 Homo sapiens
2.4.1.88 FS
-
 Canis lupus familiaris
2.4.1.88 FS
-
 Homo sapiens