BRENDA - Enzyme Database

Solubilization and partial characterization of UDP-N-acetylgalactosamine: globoside alpha-N-acetylgalactosaminyltransferase from dog spleen microsomes

Ishibashi, T.; Ohkubo, I.; Makita, A.; Biochim. Biophys. Acta 484, 24-34 (1977)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.88
diphosphatidylglycerol
activation
Canis lupus familiaris
2.4.1.88
GSH
slight stimulation
Canis lupus familiaris
2.4.1.88
additional information
requirement for phospholipids
Canis lupus familiaris
2.4.1.88
phosphatidic acid
activation
Canis lupus familiaris
2.4.1.88
phosphatidylcholine
activation
Canis lupus familiaris
2.4.1.88
phosphatidylethanolamine
activation
Canis lupus familiaris
2.4.1.88
phosphatidylinositol
activation
Canis lupus familiaris
2.4.1.88
phosphatidylserine
activation
Canis lupus familiaris
2.4.1.88
sphingomyelin
activation
Canis lupus familiaris
2.4.1.88
Triton X-100
activation; best at 0.3-0.6%
Canis lupus familiaris
General Stability
EC Number
General Stability
Organism
2.4.1.88
phosphatidylserine stabilizes
Canis lupus familiaris
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.88
ADP
-
Canis lupus familiaris
2.4.1.88
ATP
-
Canis lupus familiaris
2.4.1.88
CDP
-
Canis lupus familiaris
2.4.1.88
EDTA
strong
Canis lupus familiaris
2.4.1.88
GDP
-
Canis lupus familiaris
2.4.1.88
IDP
-
Canis lupus familiaris
2.4.1.88
additional information
no inhibitor: NaF
Canis lupus familiaris
2.4.1.88
NaN3
weak
Canis lupus familiaris
2.4.1.88
PCMB
over 90% inhibition at 2 mM
Canis lupus familiaris
2.4.1.88
UDP
85% inhibition at 1.5 mM
Canis lupus familiaris
2.4.1.88
uridine
weak
Canis lupus familiaris
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.88
0.01
-
UDP-N-acetylgalactosamine
-
Canis lupus familiaris
2.4.1.88
0.5
-
globoside
-
Canis lupus familiaris
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.88
membrane
-
Canis lupus familiaris
16020
-
2.4.1.88
microsome
-
Canis lupus familiaris
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.88
Mg2+
no effect
Canis lupus familiaris
2.4.1.88
Mn2+
absolute requirement, cannot be replaced by Ca2+ and Mg2+; optimal concentration: 10 mM
Canis lupus familiaris
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.1.88
UDP-N-acetyl-D-galactosamine + N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide
Canis lupus familiaris
involved in biosynthesis of carbohydrate moiety of sphingoglycolipids
UDP + N-acetyl-D-galactosaminyl-1,3-N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide
i.e. Forssman hapten
Canis lupus familiaris
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.1.88
Canis lupus familiaris
-
mongrel dog, adult
-
Purification (Commentary)
EC Number
Commentary
Organism
2.4.1.88
partial
Canis lupus familiaris
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.88
spleen
-
Canis lupus familiaris
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.1.88
additional information
-
-
Canis lupus familiaris
Storage Stability
EC Number
Storage Stability
Organism
2.4.1.88
-20°C, at least 2 weeks without loss of activity
Canis lupus familiaris
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.88
UDP-N-acetyl-D-galactosamine + N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide
-
489482
Canis lupus familiaris
UDP + N-acetyl-D-galactosaminyl-1,3-N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide
i.e. Forssman hapten
489482
Canis lupus familiaris
?
2.4.1.88
UDP-N-acetyl-D-galactosamine + N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide
involved in biosynthesis of carbohydrate moiety of sphingoglycolipids
489482
Canis lupus familiaris
UDP + N-acetyl-D-galactosaminyl-1,3-N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide
i.e. Forssman hapten
489482
Canis lupus familiaris
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.1.88
37
-
assay at
Canis lupus familiaris
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.88
6.6
-
-
Canis lupus familiaris
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.88
diphosphatidylglycerol
activation
Canis lupus familiaris
2.4.1.88
GSH
slight stimulation
Canis lupus familiaris
2.4.1.88
additional information
requirement for phospholipids
Canis lupus familiaris
2.4.1.88
phosphatidic acid
activation
Canis lupus familiaris
2.4.1.88
phosphatidylcholine
activation
Canis lupus familiaris
2.4.1.88
phosphatidylethanolamine
activation
Canis lupus familiaris
2.4.1.88
phosphatidylinositol
activation
Canis lupus familiaris
2.4.1.88
phosphatidylserine
activation
Canis lupus familiaris
2.4.1.88
sphingomyelin
activation
Canis lupus familiaris
2.4.1.88
Triton X-100
activation; best at 0.3-0.6%
Canis lupus familiaris
General Stability (protein specific)
EC Number
General Stability
Organism
2.4.1.88
phosphatidylserine stabilizes
Canis lupus familiaris
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.88
ADP
-
Canis lupus familiaris
2.4.1.88
ATP
-
Canis lupus familiaris
2.4.1.88
CDP
-
Canis lupus familiaris
2.4.1.88
EDTA
strong
Canis lupus familiaris
2.4.1.88
GDP
-
Canis lupus familiaris
2.4.1.88
IDP
-
Canis lupus familiaris
2.4.1.88
additional information
no inhibitor: NaF
Canis lupus familiaris
2.4.1.88
NaN3
weak
Canis lupus familiaris
2.4.1.88
PCMB
over 90% inhibition at 2 mM
Canis lupus familiaris
2.4.1.88
UDP
85% inhibition at 1.5 mM
Canis lupus familiaris
2.4.1.88
uridine
weak
Canis lupus familiaris
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.88
0.01
-
UDP-N-acetylgalactosamine
-
Canis lupus familiaris
2.4.1.88
0.5
-
globoside
-
Canis lupus familiaris
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.88
membrane
-
Canis lupus familiaris
16020
-
2.4.1.88
microsome
-
Canis lupus familiaris
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.88
Mg2+
no effect
Canis lupus familiaris
2.4.1.88
Mn2+
absolute requirement, cannot be replaced by Ca2+ and Mg2+; optimal concentration: 10 mM
Canis lupus familiaris
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.1.88
UDP-N-acetyl-D-galactosamine + N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide
Canis lupus familiaris
involved in biosynthesis of carbohydrate moiety of sphingoglycolipids
UDP + N-acetyl-D-galactosaminyl-1,3-N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide
i.e. Forssman hapten
Canis lupus familiaris
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.1.88
partial
Canis lupus familiaris
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.88
spleen
-
Canis lupus familiaris
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.1.88
additional information
-
-
Canis lupus familiaris
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.4.1.88
-20°C, at least 2 weeks without loss of activity
Canis lupus familiaris
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.88
UDP-N-acetyl-D-galactosamine + N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide
-
489482
Canis lupus familiaris
UDP + N-acetyl-D-galactosaminyl-1,3-N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide
i.e. Forssman hapten
489482
Canis lupus familiaris
?
2.4.1.88
UDP-N-acetyl-D-galactosamine + N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide
involved in biosynthesis of carbohydrate moiety of sphingoglycolipids
489482
Canis lupus familiaris
UDP + N-acetyl-D-galactosaminyl-1,3-N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide
i.e. Forssman hapten
489482
Canis lupus familiaris
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.1.88
37
-
assay at
Canis lupus familiaris
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.88
6.6
-
-
Canis lupus familiaris