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Literature summary extracted from

  • Taniguchi, N.; Yokosawa, N.; Gasa, S.; Makita, A.
    UDP-N-acetylgalactosamine:globoside alpha-3-N-acetylgalactosaminyltransferase. Purification, characterization, and some properties (1982), J. Biol. Chem., 257, 10631-10637.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.4.1.88 glycerol, 50%, stabilizes during storage Canis lupus familiaris
2.4.1.88 repeated freeze-thawing inactivates, UDP and glycerol protect Canis lupus familiaris
2.4.1.88 UDP, 0.1 mM, stabilizes during storage Canis lupus familiaris

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.88 Co2+ in the presence of Mn2+ Canis lupus familiaris
2.4.1.88 Cu2+ in the presence of Mn2+ Canis lupus familiaris
2.4.1.88 Ni2+ in the presence of Mn2+ Canis lupus familiaris
2.4.1.88 UDP competitive to UDP-N-acetylgalactosamine, non-competitive to globoside Canis lupus familiaris
2.4.1.88 Zn2+ in the presence of Mn2+ Canis lupus familiaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.88 0.00156
-
 globoside
-
 Canis lupus familiaris
2.4.1.88 0.01
-
 UDP-N-acetylgalactosamine
-
 Canis lupus familiaris

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.88 membrane
-
 Canis lupus familiaris 16020
-
2.4.1.88 microsome
-
 Canis lupus familiaris
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.88 Co2+ can replace Mn2+ with 20% efficiency, inhibits in the presence of Mn2+ Canis lupus familiaris
2.4.1.88 Cu2+ can replace Mn2+ with 31% efficiency, inhibits in the presence of Mn2+ Canis lupus familiaris
2.4.1.88 Fe2+ can replace Mn2+ with 76% efficiency Canis lupus familiaris
2.4.1.88 Mg2+ stimulation, only in the presence of Mn2+ Canis lupus familiaris
2.4.1.88 Mn2+ requirement Canis lupus familiaris
2.4.1.88 Mn2+ Km-value 0.45 mM Canis lupus familiaris
2.4.1.88 additional information no activation by Ni2+ Canis lupus familiaris
2.4.1.88 Zn2+ can replace Mn2+ with 83% efficiency Canis lupus familiaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.88 56000
-
 1 * 56000 + 1 * 60000, SDS-PAGE Canis lupus familiaris
2.4.1.88 60000
-
 1 * 56000 + 1 * 60000, SDS-PAGE Canis lupus familiaris
2.4.1.88 120000
-
 gel filtration Canis lupus familiaris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.88 UDP-N-acetyl-D-galactosamine + N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide Canis lupus familiaris
-
 UDP + N-acetyl-D-galactosaminyl-1,3-N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide i.e. Forssman hapten ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.88 Canis lupus familiaris
-
 mongrel dog, adult
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.88 affinity chromatography Canis lupus familiaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.88 spleen
-
 Canis lupus familiaris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.88 0.000059
-
 purified enzyme Canis lupus familiaris

Storage Stability

EC Number Storage Stability Organism
2.4.1.88 -80°C, 0.1 mg/ml enzyme in 25 mM sodium cacodylate buffer with 1% Triton X-100, at least 6 months Canis lupus familiaris
2.4.1.88 4°C, 0.1 mg/ml enzyme in 25 mM sodium cacodylate buffer with 1% Triton X-100, 40% loss of activity within 3 months Canis lupus familiaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.88 additional information no substrates: oligosaccharide of blood type H substance, asialo-GM1, asialo-GM2 and GD1a, galactosyl-alpha-1,4-galactosyl-beta-1,4-glucosylceramide, UDP, UDP-galactose, 2'-fucosyllactose, H blood group glycoprotein, bovine submaxillary deglycosylated mucin, GM1-3, glucosyl-beta-1,1-ceramide, galactosyl-beta-1,1-ceramide, galactosyl-beta-1,4-glucosylceramide Canis lupus familiaris ?
-
 ?
2.4.1.88 UDP-N-acetyl-alpha-D-galactosamine + globoside
-
 Canis lupus familiaris UDP + N-acetyl-D-galactosaminyl-N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide
-
 ?
2.4.1.88 UDP-N-acetyl-D-galactosamine + N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide
-
 Canis lupus familiaris UDP + N-acetyl-D-galactosaminyl-1,3-N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide i.e. Forssman hapten ?
2.4.1.88 UDP-N-acetyl-D-galactosamine + N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide i.e. globoside, catalyzes the transfer of N-acetylgalactosamine in alpha-1,3-linkage to globoside Canis lupus familiaris UDP + N-acetyl-D-galactosaminyl-1,3-N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide i.e. Forssman hapten ir
2.4.1.88 UDP-N-acetyl-D-galactosamine + N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide general structure of preferred substrates: N-acetylgalactosaminyl-beta-1,3-galactosyl-O-R Canis lupus familiaris UDP + N-acetyl-D-galactosaminyl-1,3-N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide i.e. Forssman hapten ir
2.4.1.88 UDP-N-acetyl-D-galactosamine + N-acetylgalactosaminyl-1,3-beta-galactosyl-alpha-1,3-galactosyl-beta-1,4-glucosylceramide
-
 Canis lupus familiaris UDP + N-acetyl-D-galactosamine-1,3-N-acetylgalactosaminyl-1,3-beta-galactosyl-alpha-1,3-galactosyl-beta-1,4-glucosylceramide
-
 ?
2.4.1.88 UDP-N-acetyl-D-galactosamine + oligosaccharide of globoside
-
 Canis lupus familiaris ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.88 dimer 1 * 56000 + 1 * 60000, SDS-PAGE Canis lupus familiaris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.88 37
-
 assay at Canis lupus familiaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.88 6.7
-
-
 Canis lupus familiaris

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.88 6.5 6.9
-
 Canis lupus familiaris