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Literature summary extracted from

  • Jones, P.R.; Moller, B.L.; Hoj, P.B.
    The UDP-glucose:p-hydroxymandelonitrile-O-glucosyltransferase that catalyzes the last step in synthesis of the cyanogenic glucoside dhurrin in Sorghum bicolor. Isolation, cloning, heterologous expression, and substrate specificity (1999), J. Biol. Chem., 274, 35483-35491.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.85 bovine serum albumin activates Sorghum bicolor
2.4.1.85 dithiothreitol requirement, 5 mM Sorghum bicolor

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.85 cloning of a full-length cDNA encoding enzyme, expression in Escherichia coli JM109, 492-amino acids translation product Sorghum bicolor

General Stability

EC Number General Stability Organism
2.4.1.85 activity is largely uneffected by both freezing at -80°C and addition of glycerol, DTT stabilizes Sorghum bicolor

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.85 4-hydroxybenzaldehyde potent inhibitor, 1 mg/ml bovine serum albumin protects Sorghum bicolor
2.4.1.85 4-hydroxymandelonitrile possibly an inhibitor, 1 mg/ml bovine serum albumin protects Sorghum bicolor
2.4.1.85 benzaldehyde potent inhibitor, 1 mg/ml bovine serum albumin protects Sorghum bicolor
2.4.1.85 Dhurrin potent inhibitor, 1 mg/ml bovine serum albumin protects Sorghum bicolor
2.4.1.85 mandelonitrile potent inhibitor, 1 mg/ml bovine serum albumin protects Sorghum bicolor
2.4.1.85 sambunigrin potent inhibitor, 1 mg/ml bovine serum albumin protects Sorghum bicolor

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.85 soluble
-
 Sorghum bicolor
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.85 52900
-
 x * 52900, calculated from the amino acid sequence Sorghum bicolor

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.85 UDPglucose + (S)-4-hydroxymandelonitrile Sorghum bicolor endogenous substrate is p-hydroxymandelonitrile, final step in the biosynthesis of dhurrin, transformation of the labile cyanohydrin into a stable storage form UDP + (S)-4-hydroxymandelonitrile beta-D-glucoside
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.85 Sorghum bicolor Q9SBL1 Pacific seeds, Australia, cultivar MR31
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.85 420fold, native and recombinant enzyme, expressed in Escherichia coli JM109 Sorghum bicolor

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.85 seedling etiolated Sorghum bicolor
-

Storage Stability

EC Number Storage Stability Organism
2.4.1.85 4°C, 2 days, lowering the concentration of DTT from 5 to 2 mM results in a 10fold decrease in activity Sorghum bicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.85 additional information exclusively specific for the presence of a benzyl group in the substrate, no substrate: acetone cyanohydrin, hydroquinone, p-hydroxybenzaldehyde, gentisic acid, caffeic acid, 2-hydroxy cinnamic acid, resveratrol, salicylic acid, p-hydroxymandelic acid, quercetin, caynidin, biochanin A, naringenin, apigenin, indole acetic acid, tomatidine Sorghum bicolor additional information
-
 ?
2.4.1.85 UDPalpha-D-glucose + benzoic acid at 4% of the rate with p-hydroxymandelonitrile Sorghum bicolor UDP + 1-O-benzoyl-beta-D-glucose
-
 ?
2.4.1.85 UDPglucose + (S)-4-hydroxymandelonitrile C-terminal region encodes the UDPglucose binding domain Sorghum bicolor UDP + (S)-4-hydroxymandelonitrile beta-D-glucoside identical with dhurrin ?
2.4.1.85 UDPglucose + (S)-4-hydroxymandelonitrile stereospecific for the S-enantiomer Sorghum bicolor UDP + (S)-4-hydroxymandelonitrile beta-D-glucoside identical with dhurrin ?
2.4.1.85 UDPglucose + (S)-4-hydroxymandelonitrile endogenous substrate is p-hydroxymandelonitrile, final step in the biosynthesis of dhurrin, transformation of the labile cyanohydrin into a stable storage form Sorghum bicolor UDP + (S)-4-hydroxymandelonitrile beta-D-glucoside
-
 ?
2.4.1.85 UDPglucose + (S)-mandelonitrile at 78% of the rate with p-hydroxymandelonitrile Sorghum bicolor UDP + (S)-mandelonitrile beta-D-glucoside
-
 ?
2.4.1.85 UDPglucose + benzyl alcohol at 13% of the rate with p-hydroxymandelonitrile Sorghum bicolor UDP + benzylglucoside
-
 ?
2.4.1.85 UDPglucose + geraniol at 11% of the rate with p-hydroxymandelonitrile Sorghum bicolor UDP + geraniol glucoside
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.85 ? x * 50000-55000, SDS-PAGE Sorghum bicolor
2.4.1.85 ? x * 52900, calculated from the amino acid sequence Sorghum bicolor

Synonyms

EC Number Synonyms Comment Organism
2.4.1.85 sbHMNGT
-
 Sorghum bicolor

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.85 30
-
 assay at Sorghum bicolor

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.85 additional information
-
 theoretical pI of 5.3 Sorghum bicolor