BRENDA - Enzyme Database

Characterization of recombinant yeast dolichyl mannosyl phosphate synthase and site-directed mutagenesis of its cysteine residues

Forsee, W.T.; McPherson, D.; Schutzbach, J.S.; Eur. J. Biochem. 244, 953-958 (1997)

Data extracted from this reference:

Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.4.1.83
C172S
specific activity similar to the wild-type enzyme
Saccharomyces cerevisiae
2.4.1.83
C259S
specific activity similar to the wild-type enzyme
Saccharomyces cerevisiae
2.4.1.83
C93S
specific activity similar to the wild-type enzyme
Saccharomyces cerevisiae
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.83
5,5'-dithiobis(2-nitrobenzoic acid)
-
Saccharomyces cerevisiae
2.4.1.83
lucifer yellow iodoacetamide
-
Saccharomyces cerevisiae
2.4.1.83
additional information
GDP, GTP, IDP or GMP provide protection against inactivation by thiol reagents
Saccharomyces cerevisiae
2.4.1.83
NEM
-
Saccharomyces cerevisiae
2.4.1.83
p-hydroxymercuribenzoate
-
Saccharomyces cerevisiae
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.1.83
Saccharomyces cerevisiae
-
recombinant enzyme
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.83
GDPmannose + dolichyl phosphate
-
489457
Saccharomyces cerevisiae
GDP + dolichyl D-mannosyl phosphate
-
489457
Saccharomyces cerevisiae
?
Subunits
EC Number
Subunits
Commentary
Organism
2.4.1.83
?
-
Saccharomyces cerevisiae
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.4.1.83
C172S
specific activity similar to the wild-type enzyme
Saccharomyces cerevisiae
2.4.1.83
C259S
specific activity similar to the wild-type enzyme
Saccharomyces cerevisiae
2.4.1.83
C93S
specific activity similar to the wild-type enzyme
Saccharomyces cerevisiae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.83
5,5'-dithiobis(2-nitrobenzoic acid)
-
Saccharomyces cerevisiae
2.4.1.83
lucifer yellow iodoacetamide
-
Saccharomyces cerevisiae
2.4.1.83
additional information
GDP, GTP, IDP or GMP provide protection against inactivation by thiol reagents
Saccharomyces cerevisiae
2.4.1.83
NEM
-
Saccharomyces cerevisiae
2.4.1.83
p-hydroxymercuribenzoate
-
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.83
GDPmannose + dolichyl phosphate
-
489457
Saccharomyces cerevisiae
GDP + dolichyl D-mannosyl phosphate
-
489457
Saccharomyces cerevisiae
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.4.1.83
?
-
Saccharomyces cerevisiae