Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zhu, B.C.; Laine, R.A.
    Dolichyl-phosphomannose synthase from the archae Thermoplasma acidophilum (1996), Glycobiology, 6, 811-816.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.83 Amphomycin
-
Thermoplasma acidophilum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.83 0.0026
-
dolichyl phosphate
-
Thermoplasma acidophilum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.83 Mg2+ stimulated by MgCl2, optimal concentration: 20 mM Thermoplasma acidophilum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.83 42000
-
x * 42000, SDS-PAGE under reducing conditions Thermoplasma acidophilum

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.83 Thermoplasma acidophilum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.83
-
Thermoplasma acidophilum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.83 additional information
-
-
Thermoplasma acidophilum

Storage Stability

EC Number Storage Stability Organism
2.4.1.83 -20°C, phosphate buffer, pH 6.0, 20 mM Mg2+, 0.2% polidocanol, 20% glycerol, stable for 2 weeks Thermoplasma acidophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.83 GDPmannose + C55-polyisoprenol
-
Thermoplasma acidophilum GDP + D-mannosyl-C55-polyisopropenol
-
?
2.4.1.83 GDPmannose + dolichyl phosphate
-
Thermoplasma acidophilum GDP + dolichyl D-mannosyl phosphate
-
?

Subunits

EC Number Subunits Comment Organism
2.4.1.83 ? x * 42000, SDS-PAGE under reducing conditions Thermoplasma acidophilum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.83 6
-
-
Thermoplasma acidophilum