BRENDA - Enzyme Database

Dolichyl-phosphomannose synthase from the archae Thermoplasma acidophilum

Zhu, B.C.; Laine, R.A.; Glycobiology 6, 811-816 (1996)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.83
Amphomycin
-
Thermoplasma acidophilum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.83
0.0026
-
dolichyl phosphate
-
Thermoplasma acidophilum
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.83
Mg2+
stimulated by MgCl2, optimal concentration: 20 mM
Thermoplasma acidophilum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.4.1.83
42000
-
x * 42000, SDS-PAGE under reducing conditions
Thermoplasma acidophilum
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.1.83
Thermoplasma acidophilum
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.4.1.83
-
Thermoplasma acidophilum
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.1.83
additional information
-
-
Thermoplasma acidophilum
Storage Stability
EC Number
Storage Stability
Organism
2.4.1.83
-20°C, phosphate buffer, pH 6.0, 20 mM Mg2+, 0.2% polidocanol, 20% glycerol, stable for 2 weeks
Thermoplasma acidophilum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.83
GDPmannose + C55-polyisoprenol
-
489456
Thermoplasma acidophilum
GDP + D-mannosyl-C55-polyisopropenol
-
-
-
?
2.4.1.83
GDPmannose + dolichyl phosphate
-
489456
Thermoplasma acidophilum
GDP + dolichyl D-mannosyl phosphate
-
489456
Thermoplasma acidophilum
?
Subunits
EC Number
Subunits
Commentary
Organism
2.4.1.83
?
x * 42000, SDS-PAGE under reducing conditions
Thermoplasma acidophilum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.83
6
-
-
Thermoplasma acidophilum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.83
Amphomycin
-
Thermoplasma acidophilum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.83
0.0026
-
dolichyl phosphate
-
Thermoplasma acidophilum
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.83
Mg2+
stimulated by MgCl2, optimal concentration: 20 mM
Thermoplasma acidophilum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.4.1.83
42000
-
x * 42000, SDS-PAGE under reducing conditions
Thermoplasma acidophilum
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.1.83
-
Thermoplasma acidophilum
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.1.83
additional information
-
-
Thermoplasma acidophilum
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.4.1.83
-20°C, phosphate buffer, pH 6.0, 20 mM Mg2+, 0.2% polidocanol, 20% glycerol, stable for 2 weeks
Thermoplasma acidophilum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.83
GDPmannose + C55-polyisoprenol
-
489456
Thermoplasma acidophilum
GDP + D-mannosyl-C55-polyisopropenol
-
-
-
?
2.4.1.83
GDPmannose + dolichyl phosphate
-
489456
Thermoplasma acidophilum
GDP + dolichyl D-mannosyl phosphate
-
489456
Thermoplasma acidophilum
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.4.1.83
?
x * 42000, SDS-PAGE under reducing conditions
Thermoplasma acidophilum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.83
6
-
-
Thermoplasma acidophilum