BRENDA - Enzyme Database

Solubilization and one-step purification of mannosylphosphodolichol synthase from Trichoderma reesei

Kruszewska, J.S.; Perlinska-Lenart, U.; Palamarczyk, G.; Acta Biochim. Pol. 43, 397-401 (1996)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.83
additional information
the enzyme is activated by cAMP-dependent protein kinase
Trichoderma reesei
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.83
membrane
-
Trichoderma reesei
16020
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.1.83
Trichoderma reesei
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
2.4.1.83
phosphoprotein
the enzyme contains a potential site for phosphorylation by cAMP-dependent protein kinase
Trichoderma reesei
Purification (Commentary)
EC Number
Commentary
Organism
2.4.1.83
one-step purification
Trichoderma reesei
Storage Stability
EC Number
Storage Stability
Organism
2.4.1.83
during solubilization the enzyme is stabilized by the presence of lipophilic substrate dilochylphosphate and phospholipids as well as by protease inhibitors
Trichoderma reesei
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.83
GDPmannose + dolichyl phosphate
-
489455
Trichoderma reesei
GDP + dolichyl D-mannosyl phosphate
-
489455
Trichoderma reesei
?
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.83
additional information
the enzyme is activated by cAMP-dependent protein kinase
Trichoderma reesei
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.83
membrane
-
Trichoderma reesei
16020
-
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
2.4.1.83
phosphoprotein
the enzyme contains a potential site for phosphorylation by cAMP-dependent protein kinase
Trichoderma reesei
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.1.83
one-step purification
Trichoderma reesei
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.4.1.83
during solubilization the enzyme is stabilized by the presence of lipophilic substrate dilochylphosphate and phospholipids as well as by protease inhibitors
Trichoderma reesei
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.83
GDPmannose + dolichyl phosphate
-
489455
Trichoderma reesei
GDP + dolichyl D-mannosyl phosphate
-
489455
Trichoderma reesei
?