BRENDA - Enzyme Database

Partial purification and characterization of dolichol phosphate mannose synthase from Entamoeba histolytica

Villagomez-Castro, J.C.; Calvo-Mendez, C.; Flores-Carreon, A.; Lopez-Romero, E.; Glycobiology 10, 1311-1316 (2000)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.83
phosphatidylcholine
increases activity 1.3fold at the optimal concentration
Entamoeba histolytica
2.4.1.83
phosphatidylethanolamine
increases activity 2.3fold at the optimal concentration
Entamoeba histolytica
2.4.1.83
phosphatidylinositol
increases activity 2.3fold at the optimal concentration
Entamoeba histolytica
General Stability
EC Number
General Stability
Organism
2.4.1.83
reducing agents positively influence stability
Entamoeba histolytica
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.83
0.005
-
GDPmannose
unphosphorylated enzyme
Entamoeba histolytica
2.4.1.83
0.0065
-
GDPmannose
phosphorylated enzyme
Entamoeba histolytica
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.1.83
GDPmannose + dolichyl phosphate
Entamoeba histolytica
essential enzyme in glycoprotein biosynthesis
GDP + dolichyl D-mannosyl phosphate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.1.83
Entamoeba histolytica
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.4.1.83
partial
Entamoeba histolytica
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.4.1.83
additional information
-
-
Entamoeba histolytica
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.83
GDPmannose + dolichyl phosphate
-
489447
Entamoeba histolytica
GDP + dolichyl D-mannosyl phosphate
-
489447
Entamoeba histolytica
?
2.4.1.83
GDPmannose + dolichyl phosphate
essential enzyme in glycoprotein biosynthesis
489447
Entamoeba histolytica
GDP + dolichyl D-mannosyl phosphate
-
-
-
?
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.83
phosphatidylcholine
increases activity 1.3fold at the optimal concentration
Entamoeba histolytica
2.4.1.83
phosphatidylethanolamine
increases activity 2.3fold at the optimal concentration
Entamoeba histolytica
2.4.1.83
phosphatidylinositol
increases activity 2.3fold at the optimal concentration
Entamoeba histolytica
General Stability (protein specific)
EC Number
General Stability
Organism
2.4.1.83
reducing agents positively influence stability
Entamoeba histolytica
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.83
0.005
-
GDPmannose
unphosphorylated enzyme
Entamoeba histolytica
2.4.1.83
0.0065
-
GDPmannose
phosphorylated enzyme
Entamoeba histolytica
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.1.83
GDPmannose + dolichyl phosphate
Entamoeba histolytica
essential enzyme in glycoprotein biosynthesis
GDP + dolichyl D-mannosyl phosphate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.1.83
partial
Entamoeba histolytica
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.4.1.83
additional information
-
-
Entamoeba histolytica
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.83
GDPmannose + dolichyl phosphate
-
489447
Entamoeba histolytica
GDP + dolichyl D-mannosyl phosphate
-
489447
Entamoeba histolytica
?
2.4.1.83
GDPmannose + dolichyl phosphate
essential enzyme in glycoprotein biosynthesis
489447
Entamoeba histolytica
GDP + dolichyl D-mannosyl phosphate
-
-
-
?