BRENDA - Enzyme Database

The purification and characterization of recombinant yeast dolichyl-phosphate-mannose synthase. Site-directed mutagenesis of the putative dolichol recognition sequence

Schutzbach, J.S.; Zimmerman, J.W.; Forsee, W.T.; J. Biol. Chem. 268, 24190-24196 (1993)

Data extracted from this reference:

Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.4.1.83
I253N
higher value for the apparent Km-value for dolichyl phosphate when assayed in detergent solution, mutation has no effect on Km-value when the enzyme is reconstituted with phosphatidylethanolamine
Saccharomyces cerevisiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.83
0.0015
-
GDPmannose
wild-type enzyme assayed in solution of Nonidet P-40
Saccharomyces cerevisiae
2.4.1.83
0.0025
-
dolichyl phosphate
wild-type enzyme reconstituted with phosphatidylethanolamine
Saccharomyces cerevisiae
2.4.1.83
0.0027
-
dolichyl phosphate
wild-type enzyme reconstituted with phosphatidylethanolamine
Saccharomyces cerevisiae
2.4.1.83
0.0106
-
dolichyl phosphate
wild-type enzyme assayed in solution of Nonidet P-40
Saccharomyces cerevisiae
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.1.83
Saccharomyces cerevisiae
-
recombinant enzyme
-
Purification (Commentary)
EC Number
Commentary
Organism
2.4.1.83
-
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.83
GDPmannose + dolichyl phosphate
-
489442
Saccharomyces cerevisiae
GDP + dolichyl D-mannosyl phosphate
-
489442
Saccharomyces cerevisiae
-
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.4.1.83
I253N
higher value for the apparent Km-value for dolichyl phosphate when assayed in detergent solution, mutation has no effect on Km-value when the enzyme is reconstituted with phosphatidylethanolamine
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.83
0.0015
-
GDPmannose
wild-type enzyme assayed in solution of Nonidet P-40
Saccharomyces cerevisiae
2.4.1.83
0.0025
-
dolichyl phosphate
wild-type enzyme reconstituted with phosphatidylethanolamine
Saccharomyces cerevisiae
2.4.1.83
0.0027
-
dolichyl phosphate
wild-type enzyme reconstituted with phosphatidylethanolamine
Saccharomyces cerevisiae
2.4.1.83
0.0106
-
dolichyl phosphate
wild-type enzyme assayed in solution of Nonidet P-40
Saccharomyces cerevisiae
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.1.83
-
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.83
GDPmannose + dolichyl phosphate
-
489442
Saccharomyces cerevisiae
GDP + dolichyl D-mannosyl phosphate
-
489442
Saccharomyces cerevisiae
-