BRENDA - Enzyme Database

Solubilization and properties of polyprenyl phosphate: GDP-D-mannose mannosyl transferase

Carlo, P.L.; Villemez, C.L.; Arch. Biochem. Biophys. 198, 117-123 (1979)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.83
CuSO4
-
Acanthamoeba castellanii
2.4.1.83
GDP
0.01 mM, 81% inhibition
Acanthamoeba castellanii
2.4.1.83
GDP-D-glucose
0.01 mM, 50% inhibition
Acanthamoeba castellanii
2.4.1.83
GMP
1 mM, 84% inhibition; competitive
Acanthamoeba castellanii
2.4.1.83
HgCl2
-
Acanthamoeba castellanii
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.83
0.00025
-
GDPmannose
-
Acanthamoeba castellanii
2.4.1.83
0.0033
-
dolichyl phosphate
-
Acanthamoeba castellanii
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.83
Co2+
less efficient in activation than Mn2+
Acanthamoeba castellanii
2.4.1.83
Mg2+
less efficient than Mn2+ in activation
Acanthamoeba castellanii
2.4.1.83
Mn2+
divalent cation required, maximal activity with Mn2+
Acanthamoeba castellanii
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.1.83
Acanthamoeba castellanii
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.83
cell culture
; encysting
Acanthamoeba castellanii
-
2.4.1.83
cyst
-
Acanthamoeba castellanii
-
Storage Stability
EC Number
Storage Stability
Organism
2.4.1.83
-20°C, loss of a third of the activity after 2 weeks
Acanthamoeba castellanii
2.4.1.83
-70°C, 2 weeks, no loss of activity
Acanthamoeba castellanii
2.4.1.83
0°C, 3 days, 50% loss of activity
Acanthamoeba castellanii
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.83
GDPmannose + dolichyl phosphate
-
489440
Acanthamoeba castellanii
GDP + dolichyl D-mannosyl phosphate
-
489440
Acanthamoeba castellanii
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.1.83
27
-
-
Acanthamoeba castellanii
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
2.4.1.83
15
35
15°C: about 60% of maximal activity, 35°C: about 30% of maximal activity
Acanthamoeba castellanii
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.83
7
-
-
Acanthamoeba castellanii
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.4.1.83
5.8
8
pH 5.8: about 35% of maximal activity, pH 8.0: about 60% of maximal activity
Acanthamoeba castellanii
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.83
CuSO4
-
Acanthamoeba castellanii
2.4.1.83
GDP
0.01 mM, 81% inhibition
Acanthamoeba castellanii
2.4.1.83
GDP-D-glucose
0.01 mM, 50% inhibition
Acanthamoeba castellanii
2.4.1.83
GMP
1 mM, 84% inhibition; competitive
Acanthamoeba castellanii
2.4.1.83
HgCl2
-
Acanthamoeba castellanii
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.83
0.00025
-
GDPmannose
-
Acanthamoeba castellanii
2.4.1.83
0.0033
-
dolichyl phosphate
-
Acanthamoeba castellanii
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.83
Co2+
less efficient in activation than Mn2+
Acanthamoeba castellanii
2.4.1.83
Mg2+
less efficient than Mn2+ in activation
Acanthamoeba castellanii
2.4.1.83
Mn2+
divalent cation required, maximal activity with Mn2+
Acanthamoeba castellanii
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.83
cell culture
; encysting
Acanthamoeba castellanii
-
2.4.1.83
cyst
-
Acanthamoeba castellanii
-
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.4.1.83
-20°C, loss of a third of the activity after 2 weeks
Acanthamoeba castellanii
2.4.1.83
-70°C, 2 weeks, no loss of activity
Acanthamoeba castellanii
2.4.1.83
0°C, 3 days, 50% loss of activity
Acanthamoeba castellanii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.83
GDPmannose + dolichyl phosphate
-
489440
Acanthamoeba castellanii
GDP + dolichyl D-mannosyl phosphate
-
489440
Acanthamoeba castellanii
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.1.83
27
-
-
Acanthamoeba castellanii
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
2.4.1.83
15
35
15°C: about 60% of maximal activity, 35°C: about 30% of maximal activity
Acanthamoeba castellanii
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.83
7
-
-
Acanthamoeba castellanii
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.4.1.83
5.8
8
pH 5.8: about 35% of maximal activity, pH 8.0: about 60% of maximal activity
Acanthamoeba castellanii