BRENDA - Enzyme Database

GDPmannose dolicholphosphate mannosyltransferase of chicken liver mitochondria

Tomita, Y.; Motokawa, Y.; Biochim. Biophys. Acta 842, 176-183 (1985)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
2.4.1.83
in combination dolichyl phosphate and phospholipids are effective in stabilization
Gallus gallus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.83
0.00043
-
GDPmannose
-
Gallus gallus
2.4.1.83
0.0143
-
dolichyl phosphate
-
Gallus gallus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.83
Mg2+
optimal stimulation at 3 mM
Gallus gallus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.1.83
Gallus gallus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.4.1.83
-
Gallus gallus
Reaction
EC Number
Reaction
Commentary
Organism
2.4.1.83
GDP-alpha-D-mannose + dolichyl phosphate = GDP + dolichyl beta-D-mannosyl phosphate
sequential mechanism
Gallus gallus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.83
liver
-
Gallus gallus
-
Storage Stability
EC Number
Storage Stability
Organism
2.4.1.83
-40C, stable for about a month with 20% loss of activity
Gallus gallus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.83
GDPmannose + dolichyl phosphate
-
489439
Gallus gallus
GDP + dolichyl D-mannosyl phosphate
-
489439
Gallus gallus
?
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2.4.1.83
30
-
20 min, complete inactivation, in presence of mitochondrial extract 40% loss of activity, stable for 30 min in presence of sphingomyelin and dolichyl phosphate
Gallus gallus
2.4.1.83
40
-
20 min, 50% loss of activity in presence of sphingomyelin and dolichyl phosphate
Gallus gallus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.83
7.2
-
-
Gallus gallus
General Stability (protein specific)
EC Number
General Stability
Organism
2.4.1.83
in combination dolichyl phosphate and phospholipids are effective in stabilization
Gallus gallus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.83
0.00043
-
GDPmannose
-
Gallus gallus
2.4.1.83
0.0143
-
dolichyl phosphate
-
Gallus gallus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.83
Mg2+
optimal stimulation at 3 mM
Gallus gallus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.1.83
-
Gallus gallus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.83
liver
-
Gallus gallus
-
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.4.1.83
-40C, stable for about a month with 20% loss of activity
Gallus gallus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.83
GDPmannose + dolichyl phosphate
-
489439
Gallus gallus
GDP + dolichyl D-mannosyl phosphate
-
489439
Gallus gallus
?
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2.4.1.83
30
-
20 min, complete inactivation, in presence of mitochondrial extract 40% loss of activity, stable for 30 min in presence of sphingomyelin and dolichyl phosphate
Gallus gallus
2.4.1.83
40
-
20 min, 50% loss of activity in presence of sphingomyelin and dolichyl phosphate
Gallus gallus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.83
7.2
-
-
Gallus gallus