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Literature summary extracted from

  • Tomita, Y.; Motokawa, Y.
    GDPmannose dolicholphosphate mannosyltransferase of chicken liver mitochondria (1985), Biochim. Biophys. Acta, 842, 176-183.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.4.1.83 in combination dolichyl phosphate and phospholipids are effective in stabilization Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.83 0.00043
-
 GDPmannose
-
 Gallus gallus
2.4.1.83 0.0143
-
 dolichyl phosphate
-
 Gallus gallus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.83 Mg2+ optimal stimulation at 3 mM Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.83 Gallus gallus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.83
-
 Gallus gallus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.83 GDP-alpha-D-mannose + dolichyl phosphate = GDP + dolichyl beta-D-mannosyl phosphate sequential mechanism Gallus gallus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.83 liver
-
 Gallus gallus
-

Storage Stability

EC Number Storage Stability Organism
2.4.1.83 -40°C, stable for about a month with 20% loss of activity Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.83 GDPmannose + dolichyl phosphate
-
 Gallus gallus GDP + dolichyl D-mannosyl phosphate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.83 30
-
 20 min, complete inactivation, in presence of mitochondrial extract 40% loss of activity, stable for 30 min in presence of sphingomyelin and dolichyl phosphate Gallus gallus
2.4.1.83 40
-
 20 min, 50% loss of activity in presence of sphingomyelin and dolichyl phosphate Gallus gallus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.83 7.2
-
-
 Gallus gallus