BRENDA - Enzyme Database

Purification and properties of a mannosyltransferase solubilized from mitochondrial outer membranes

Gasnier, F.; Morelis, R.; Louisot, P.; Gateau, O.; Biochim. Biophys. Acta 925, 297-304 (1987)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.83
lysophosphatidylcholine
activates
Mus musculus
2.4.1.83
phosphatidylcholine
activates
Mus musculus
2.4.1.83
sphingomyelin
activates
Mus musculus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.83
GDP
0.1 mM, 75% inhibition
Mus musculus
2.4.1.83
GMP
1 mM, 75% inhibition
Mus musculus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.83
0.00133
-
GDPmannose
-
Mus musculus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.83
mitochondrion
outer membrane
Mus musculus
5739
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.83
Mg2+
stimulation by addition of divalent cations, maximal stimulation at 5 mM
Mus musculus
2.4.1.83
Mn2+
stimulation by divalent cation, Mn2+ is less effective than Mg2+
Mus musculus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.1.83
Mus musculus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.4.1.83
-
Mus musculus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.83
liver
-
Mus musculus
-
Storage Stability
EC Number
Storage Stability
Organism
2.4.1.83
4°C, enzyme is relatively stable when stored overnight in phosphate buffer and retains full activity when stored within liposomes
Mus musculus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.83
GDPmannose + dolichyl phosphate
-
489438
Mus musculus
GDP + dolichyl D-mannosyl phosphate
-
489438
Mus musculus
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.83
6.1
-
Mes buffer or sodium/potassium phosphate buffer
Mus musculus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.83
lysophosphatidylcholine
activates
Mus musculus
2.4.1.83
phosphatidylcholine
activates
Mus musculus
2.4.1.83
sphingomyelin
activates
Mus musculus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.83
GDP
0.1 mM, 75% inhibition
Mus musculus
2.4.1.83
GMP
1 mM, 75% inhibition
Mus musculus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.83
0.00133
-
GDPmannose
-
Mus musculus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.83
mitochondrion
outer membrane
Mus musculus
5739
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.83
Mg2+
stimulation by addition of divalent cations, maximal stimulation at 5 mM
Mus musculus
2.4.1.83
Mn2+
stimulation by divalent cation, Mn2+ is less effective than Mg2+
Mus musculus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.1.83
-
Mus musculus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.83
liver
-
Mus musculus
-
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.4.1.83
4°C, enzyme is relatively stable when stored overnight in phosphate buffer and retains full activity when stored within liposomes
Mus musculus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.83
GDPmannose + dolichyl phosphate
-
489438
Mus musculus
GDP + dolichyl D-mannosyl phosphate
-
489438
Mus musculus
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.83
6.1
-
Mes buffer or sodium/potassium phosphate buffer
Mus musculus