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Literature summary extracted from

  • Gasnier, F.; Morelis, R.; Louisot, P.; Gateau, O.
    Purification and properties of a mannosyltransferase solubilized from mitochondrial outer membranes (1987), Biochim. Biophys. Acta, 925, 297-304.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.83 lysophosphatidylcholine activates Mus musculus
2.4.1.83 phosphatidylcholine activates Mus musculus
2.4.1.83 sphingomyelin activates Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.83 GDP 0.1 mM, 75% inhibition Mus musculus
2.4.1.83 GMP 1 mM, 75% inhibition Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.83 0.00133
-
 GDPmannose
-
 Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.83 mitochondrion outer membrane Mus musculus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.83 Mg2+ stimulation by addition of divalent cations, maximal stimulation at 5 mM Mus musculus
2.4.1.83 Mn2+ stimulation by divalent cation, Mn2+ is less effective than Mg2+ Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.83 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.83
-
 Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.83 liver
-
 Mus musculus
-

Storage Stability

EC Number Storage Stability Organism
2.4.1.83 4°C, enzyme is relatively stable when stored overnight in phosphate buffer and retains full activity when stored within liposomes Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.83 GDPmannose + dolichyl phosphate
-
 Mus musculus GDP + dolichyl D-mannosyl phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.83 6.1
-
 Mes buffer or sodium/potassium phosphate buffer Mus musculus