BRENDA - Enzyme Database

Characterization of mannosyl-transfer reactions catalyzed by dolichyl-mannosyl-phosphate-synthase

Jensen, J.W.; Schutzbach, J.S.; Carbohydr. Res. 149, 199-208 (1986)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.83
Phospholipid
no absolute requirement for phospholipid, the phospholipid is required for interaction of the enzyme with the long chain polyisoprenol substrate dolichyl phosphate
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.83
Amphomycin
0.03 mg/ml, 50% inhibition
Rattus norvegicus
2.4.1.83
GDP
0.0013 mM, 50% inhibition
Rattus norvegicus
2.4.1.83
GMP
0.016 mM, 50% inhibition
Rattus norvegicus
2.4.1.83
GTP
0.003 mM, 50% inhibition
Rattus norvegicus
2.4.1.83
Nonidet P-40
0.01%, 50% inhibition
Rattus norvegicus
2.4.1.83
p-hydroxymercuribenzoate
0.003 mM, 50% inhibition
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.83
0.0003
-
dolichyl phosphate
-
Rattus norvegicus
2.4.1.83
0.00069
-
GDPmannose
-
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.83
microsome
-
Rattus norvegicus
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.83
Co2+
5 mM, divalent metal required, 90% of the activation with 5 mM Mn2+
Rattus norvegicus
2.4.1.83
Mg2+
5 mM, divalent metal required, 70% of the activation with 5 mM Mn2+
Rattus norvegicus
2.4.1.83
Mn2+
5 mM, divalent cation required, highest activation with Mn2+
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.1.83
Rattus norvegicus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.83
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.83
GDPmannose + dolichyl phosphate
-
489431
Rattus norvegicus
GDP + dolichyl D-mannosyl phosphate
-
489431
Rattus norvegicus
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.83
7.5
-
-
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.83
Phospholipid
no absolute requirement for phospholipid, the phospholipid is required for interaction of the enzyme with the long chain polyisoprenol substrate dolichyl phosphate
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.83
Amphomycin
0.03 mg/ml, 50% inhibition
Rattus norvegicus
2.4.1.83
GDP
0.0013 mM, 50% inhibition
Rattus norvegicus
2.4.1.83
GMP
0.016 mM, 50% inhibition
Rattus norvegicus
2.4.1.83
GTP
0.003 mM, 50% inhibition
Rattus norvegicus
2.4.1.83
Nonidet P-40
0.01%, 50% inhibition
Rattus norvegicus
2.4.1.83
p-hydroxymercuribenzoate
0.003 mM, 50% inhibition
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.83
0.0003
-
dolichyl phosphate
-
Rattus norvegicus
2.4.1.83
0.00069
-
GDPmannose
-
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.83
microsome
-
Rattus norvegicus
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.83
Co2+
5 mM, divalent metal required, 90% of the activation with 5 mM Mn2+
Rattus norvegicus
2.4.1.83
Mg2+
5 mM, divalent metal required, 70% of the activation with 5 mM Mn2+
Rattus norvegicus
2.4.1.83
Mn2+
5 mM, divalent cation required, highest activation with Mn2+
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.83
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.83
GDPmannose + dolichyl phosphate
-
489431
Rattus norvegicus
GDP + dolichyl D-mannosyl phosphate
-
489431
Rattus norvegicus
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.83
7.5
-
-
Rattus norvegicus