BRENDA - Enzyme Database

Yeast mannosyl transferases requiring dolichyl phosphate and dolichyl phosphate mannose as substrate. Partial purification and characterization of the solubilized enzyme

Babczinski, P.; Haselbeck, A.; Tanner, W.; Eur. J. Biochem. 105, 509-515 (1980)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.109
deoxycholate
0.025%, stimulates slightly
Saccharomyces cerevisiae
2.4.1.109
additional information
not affected by Triton X-100 up to 0.2%
Saccharomyces cerevisiae
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.109
additional information
not affected by Triton X-100 up to 0.2%
Saccharomyces cerevisiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.83
0.007
-
GDPmannose
-
Saccharomyces cerevisiae
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.83
membrane
bound to
Saccharomyces cerevisiae
16020
-
2.4.1.109
membrane
bound
Saccharomyces cerevisiae
16020
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.83
Mg2+
Mg2+ and Mn2+ up to a concentration of 5 mM result in the same activation
Saccharomyces cerevisiae
2.4.1.83
Mn2+
Mg2+ and Mn2+ up to a concentration of 5 mM result in the same activation
Saccharomyces cerevisiae
2.4.1.109
Mg2+
Mg2+ or Mn2+ stimulates; optimal stimulation at 7 mM
Saccharomyces cerevisiae
2.4.1.109
Mn2+
Mg2+ or Mn2+ stimulates
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.1.109
dolichyl phosphate D-mannose + protein
Saccharomyces cerevisiae
production of cell-wall mannoproteins
dolichyl phosphate + O-D-mannosylprotein
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.1.83
Saccharomyces cerevisiae
-
-
-
2.4.1.109
Saccharomyces cerevisiae
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.4.1.83
-
Saccharomyces cerevisiae
2.4.1.109
partial
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.1.83
0.0253
-
-
Saccharomyces cerevisiae
2.4.1.109
0.00057
-
partially purified enzyme
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.83
GDPmannose + dolichyl phosphate
-
489426
Saccharomyces cerevisiae
GDP + dolichyl D-mannosyl phosphate
-
489426
Saccharomyces cerevisiae
?
2.4.1.109
dolichyl phosphate D-mannose + protein
-
489426
Saccharomyces cerevisiae
dolichyl phosphate + O-D-mannosylprotein
-
489426
Saccharomyces cerevisiae
?
2.4.1.109
dolichyl phosphate D-mannose + protein
the enzyme transfers mannosyl residues to the hydroxyl of serine or threonine residues
489426
Saccharomyces cerevisiae
dolichyl phosphate + O-D-mannosylprotein
-
489426
Saccharomyces cerevisiae
ir
2.4.1.109
dolichyl phosphate D-mannose + protein
acceptor substrate: HCl-treated cell wall mannoprotein from Saccharomyces cerevisiae
489426
Saccharomyces cerevisiae
dolichyl phosphate + O-D-mannosylprotein
-
489426
Saccharomyces cerevisiae
ir
2.4.1.109
dolichyl phosphate D-mannose + protein
production of cell-wall mannoproteins
489426
Saccharomyces cerevisiae
dolichyl phosphate + O-D-mannosylprotein
-
-
-
-
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.83
7.3
-
-
Saccharomyces cerevisiae
2.4.1.109
5.7
-
-
Saccharomyces cerevisiae
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.109
deoxycholate
0.025%, stimulates slightly
Saccharomyces cerevisiae
2.4.1.109
additional information
not affected by Triton X-100 up to 0.2%
Saccharomyces cerevisiae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.109
additional information
not affected by Triton X-100 up to 0.2%
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.83
0.007
-
GDPmannose
-
Saccharomyces cerevisiae
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.83
membrane
bound to
Saccharomyces cerevisiae
16020
-
2.4.1.109
membrane
bound
Saccharomyces cerevisiae
16020
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.83
Mg2+
Mg2+ and Mn2+ up to a concentration of 5 mM result in the same activation
Saccharomyces cerevisiae
2.4.1.83
Mn2+
Mg2+ and Mn2+ up to a concentration of 5 mM result in the same activation
Saccharomyces cerevisiae
2.4.1.109
Mg2+
Mg2+ or Mn2+ stimulates; optimal stimulation at 7 mM
Saccharomyces cerevisiae
2.4.1.109
Mn2+
Mg2+ or Mn2+ stimulates
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.1.109
dolichyl phosphate D-mannose + protein
Saccharomyces cerevisiae
production of cell-wall mannoproteins
dolichyl phosphate + O-D-mannosylprotein
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.1.83
-
Saccharomyces cerevisiae
2.4.1.109
partial
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.1.83
0.0253
-
-
Saccharomyces cerevisiae
2.4.1.109
0.00057
-
partially purified enzyme
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.83
GDPmannose + dolichyl phosphate
-
489426
Saccharomyces cerevisiae
GDP + dolichyl D-mannosyl phosphate
-
489426
Saccharomyces cerevisiae
?
2.4.1.109
dolichyl phosphate D-mannose + protein
-
489426
Saccharomyces cerevisiae
dolichyl phosphate + O-D-mannosylprotein
-
489426
Saccharomyces cerevisiae
?
2.4.1.109
dolichyl phosphate D-mannose + protein
the enzyme transfers mannosyl residues to the hydroxyl of serine or threonine residues
489426
Saccharomyces cerevisiae
dolichyl phosphate + O-D-mannosylprotein
-
489426
Saccharomyces cerevisiae
ir
2.4.1.109
dolichyl phosphate D-mannose + protein
acceptor substrate: HCl-treated cell wall mannoprotein from Saccharomyces cerevisiae
489426
Saccharomyces cerevisiae
dolichyl phosphate + O-D-mannosylprotein
-
489426
Saccharomyces cerevisiae
ir
2.4.1.109
dolichyl phosphate D-mannose + protein
production of cell-wall mannoproteins
489426
Saccharomyces cerevisiae
dolichyl phosphate + O-D-mannosylprotein
-
-
-
-
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.83
7.3
-
-
Saccharomyces cerevisiae
2.4.1.109
5.7
-
-
Saccharomyces cerevisiae