Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Babczinski, P.; Haselbeck, A.; Tanner, W.
    Yeast mannosyl transferases requiring dolichyl phosphate and dolichyl phosphate mannose as substrate. Partial purification and characterization of the solubilized enzyme (1980), Eur. J. Biochem., 105, 509-515.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.109 deoxycholate 0.025%, stimulates slightly Saccharomyces cerevisiae
2.4.1.109 additional information not affected by Triton X-100 up to 0.2% Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.109 additional information not affected by Triton X-100 up to 0.2% Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.83 0.007
-
 GDPmannose
-
 Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.83 membrane bound to Saccharomyces cerevisiae 16020
-
2.4.1.109 membrane bound Saccharomyces cerevisiae 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.83 Mg2+ Mg2+ and Mn2+ up to a concentration of 5 mM result in the same activation Saccharomyces cerevisiae
2.4.1.83 Mn2+ Mg2+ and Mn2+ up to a concentration of 5 mM result in the same activation Saccharomyces cerevisiae
2.4.1.109 Mg2+ Mg2+ or Mn2+ stimulates Saccharomyces cerevisiae
2.4.1.109 Mg2+ optimal stimulation at 7 mM Saccharomyces cerevisiae
2.4.1.109 Mn2+ Mg2+ or Mn2+ stimulates Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.109 dolichyl phosphate D-mannose + protein Saccharomyces cerevisiae production of cell-wall mannoproteins dolichyl phosphate + O-D-mannosylprotein
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.83 Saccharomyces cerevisiae
-
-
-
2.4.1.109 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.83
-
 Saccharomyces cerevisiae
2.4.1.109 partial Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.83 0.0253
-
-
 Saccharomyces cerevisiae
2.4.1.109 0.00057
-
 partially purified enzyme Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.83 GDPmannose + dolichyl phosphate
-
 Saccharomyces cerevisiae GDP + dolichyl D-mannosyl phosphate
-
 ?
2.4.1.109 dolichyl phosphate D-mannose + protein
-
 Saccharomyces cerevisiae dolichyl phosphate + O-D-mannosylprotein
-
 ?
2.4.1.109 dolichyl phosphate D-mannose + protein the enzyme transfers mannosyl residues to the hydroxyl of serine or threonine residues Saccharomyces cerevisiae dolichyl phosphate + O-D-mannosylprotein
-
 ir
2.4.1.109 dolichyl phosphate D-mannose + protein acceptor substrate: HCl-treated cell wall mannoprotein from Saccharomyces cerevisiae Saccharomyces cerevisiae dolichyl phosphate + O-D-mannosylprotein
-
 ir
2.4.1.109 dolichyl phosphate D-mannose + protein production of cell-wall mannoproteins Saccharomyces cerevisiae dolichyl phosphate + O-D-mannosylprotein
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.83 7.3
-
-
 Saccharomyces cerevisiae
2.4.1.109 5.7
-
-
 Saccharomyces cerevisiae