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Literature summary extracted from

  • Saito, M.; Fukushima, Y.; Tatsumi, K.; Bei, L.; Fujiki, Y.; Iwamori, M.; Igarashi, T.; Sakakihara, Y.
    Molecular cloning of Chinese hamster ceramide glucosyltransferase and its enhanced expression in peroxisome-defective mutant Z65 cells (2002), Arch. Biochem. Biophys., 403, 171-178.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.80 cloning from CHO cell line K1, overexpression in GM3-mutant CHO cell line Z65, DNA and amino acid sequence determination Cricetulus griseus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.80 D,L-threo-1-phenyl-2-(decanoylamino)-3-morpholino-1-propanol-HCl accumulation of ceramide in the cells, higher amount in Z65 mutant than in strain K1 Cricetulus griseus
2.4.1.80 D-threo-1-phenyl-2-(decanoylamino)-3-morpholino-1-propanol-HCl accumulation of ceramide in the cells, higher amount in Z65 mutant than in strain K1 Cricetulus griseus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.80 additional information
-
 amino acid sequence Cricetulus griseus
2.4.1.80 additional information
-
 signal anchor domain, glycosylation site Cricetulus griseus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.80 UDP-glucose + N-acylsphingosine Cricetulus griseus
-
 UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.80 Cricetulus griseus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.80 CHO cell strain K1, and GM3-mutant strain Z65 Cricetulus griseus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.80 0.00000034
-
 CHO cell line mutant Z65 Cricetulus griseus
2.4.1.80 0.00000139
-
 CHO cell line K-1 Cricetulus griseus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.80 UDP-glucose + N-acylsphingosine
-
 Cricetulus griseus UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?
2.4.1.80 UDP-glucose + N-acylsphingosine i.e. ceramide Cricetulus griseus UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.80 CGT
-
 Cricetulus griseus