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Literature summary extracted from

  • Matsuo, N.; Nomura, T.; Imokawa, G.
    A rapid and simple assay method for UDP-glucose:ceramide glucosyltransferase (1992), Biochim. Biophys. Acta, 1116, 97-103.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.80 CHAPS i.e. 3-[(3-cholamidopropyl)dimethylamonio]-1propanesulfonate Rattus norvegicus
2.4.1.80 detergent activity is reduced by most of the detergents tested, only CHAPS and CHAPSO at concentration of 1% w/v stimulate Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.80 Cd2+
-
 Rattus norvegicus
2.4.1.80 Zn2+
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.80 0.0087
-
 UDP-glucose
-
 Rattus norvegicus
2.4.1.80 0.292
-
 ceramide
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.80 membrane
-
 Rattus norvegicus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.80 Ba2+ activates Rattus norvegicus
2.4.1.80 Mg2+ stimulates Rattus norvegicus
2.4.1.80 Mn2+ stimulates Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.80 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.80 brain
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.80 additional information
-
 assay method development Rattus norvegicus
2.4.1.80 0.00031
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.80 UDP-glucose + N-acylsphingosine i.e. ceramide Rattus norvegicus UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.80 6.4 6.5 MES or Tris-maleate buffer Rattus norvegicus