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Literature summary extracted from

  • Taniguchi, N.; Makita, A.
    Purification and characterization of UDP-N-acetylgalactosamine: globotriaosylceramide beta-3-N-acetylgalactosaminyltransferase, a synthase of human blood group P antigen, from canine spleen (1984), J. Biol. Chem., 259, 5637-5642.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.79 dithiothreitol 10 mM, stimulation to 120% of the original activity Canis lupus familiaris

General Stability

EC Number General Stability Organism
2.4.1.79 diluted enzyme solutions, below 0.1 mg protein/ml, unstable Canis lupus familiaris
2.4.1.79 Stable to repeated freeze-thawing Canis lupus familiaris

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.79 4-hydroxymercuribenzoate 0.5 mM, complete inhibition Canis lupus familiaris
2.4.1.79 EDTA
-
Canis lupus familiaris
2.4.1.79 iodoacetamide 1 mM, 65% inhibition Canis lupus familiaris
2.4.1.79 iodoacetic acid 1 mM, 91% inhibition Canis lupus familiaris
2.4.1.79 NEM 1 mM, 67% inhibition Canis lupus familiaris
2.4.1.79 UDP competitive to UDP-N-acetylgalactosamine, non-competitive to globotriaosylceramide Canis lupus familiaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.79 0.0025
-
D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide
-
Canis lupus familiaris
2.4.1.79 0.014
-
UDP-N-acetyl-alpha-D-glucosamine
-
Canis lupus familiaris

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.79 microsome
-
Canis lupus familiaris
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.79 Co2+ 43% of the activation with Mn2+ Canis lupus familiaris
2.4.1.79 Fe2+ 19% of the activation with Mn2+ Canis lupus familiaris
2.4.1.79 Li+ 11% of the activation with Mn2+ Canis lupus familiaris
2.4.1.79 Mn2+ required Canis lupus familiaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.79 120000
-
x * 120000, SDS-PAGE without prior reduction Canis lupus familiaris

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.79 Canis lupus familiaris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.79
-
Canis lupus familiaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.79 spleen
-
Canis lupus familiaris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.79 0.000565
-
-
Canis lupus familiaris

Storage Stability

EC Number Storage Stability Organism
2.4.1.79 -80°C, above 0.1 mg protein/ml in 25 mM sodium cacodylate buffer, pH 6.9, 1% Triton X-100 and 20% glycerol, stable for at least 3 months Canis lupus familiaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.79 additional information the enzyme catalyzes the conversion of human blood group Pk antigen into P antigen Canis lupus familiaris ?
-
?
2.4.1.79 UDP-N-acetyl-alpha-D-glucosamine + globotriaosylceramide
-
Canis lupus familiaris ?
-
?
2.4.1.79 UDP-N-acetyl-D-galactosamine + D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide UDP-N-acetyl-D-galactosamine is the sole sugar donor Canis lupus familiaris UDP + N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide
-
?

Subunits

EC Number Subunits Comment Organism
2.4.1.79 ? x * 120000, SDS-PAGE without prior reduction Canis lupus familiaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.79 6.9
-
-
Canis lupus familiaris