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Literature summary extracted from

  • Hahlbrock, K.; Conn, E.E.
    The biosynthesis of cyanogenic glycosides in higher plants. I. Purification and properties of a uridine diphosphate-glucose-ketone cyanohydrin beta-glucosyltransferase from Linum usitatissimum L (1970), J. Biol. Chem., 245, 917-922.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.63 additional information no requirement for 2-mercaptoethanol or dithiothreitol, 0.01-100 mM Linum usitatissimum

General Stability

EC Number General Stability Organism
2.4.1.63 2-mercaptoethanol stabilizes during purification Linum usitatissimum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.63 MnCl2
-
Linum usitatissimum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.63 additional information no requirement for Mg2+ Linum usitatissimum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.63 UDPglucose + acetone cyanohydrin Linum usitatissimum pathway in cyanogenic glucoside biosynthesis UDP + linamarin
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.63 Linum usitatissimum
-
flax
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.63 MnCl2, ammonium sulfate, DEAE-cellulose, Sephadex G-100 Linum usitatissimum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.63 seedling
-
Linum usitatissimum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.63 0.0238
-
-
Linum usitatissimum

Storage Stability

EC Number Storage Stability Organism
2.4.1.63 glycerol, ethanol, ammonium sulfate, DTT or albumin does not stabilize during storage at -18°C or room temperature Linum usitatissimum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.63 UDPglucose + 2-hydroxy-2-ethylbutanenitrile
-
Linum usitatissimum UDP + 2-(beta-D-glucopyranosyloxy)-2-ethylbutanenitrile
-
?
2.4.1.63 UDPglucose + 2-hydroxy-2-methylbutanenitrile
-
Linum usitatissimum UDP + 2-(beta-D-glucopyranosyloxy)-2-methylbutanenitrile
-
?
2.4.1.63 UDPglucose + 2-hydroxy-2-methylpropanenitrile i.e. acetone cyanohydrin, the cyanohydrins of butanone and pentan-3-one may also be substrates, strict donor specificity, no activity with ADPglucose, CDPglucose, GDPglucose, IDPglucose or TDPglucose Linum usitatissimum UDP + linamarin
-
?
2.4.1.63 UDPglucose + acetone cyanohydrin pathway in cyanogenic glucoside biosynthesis Linum usitatissimum UDP + linamarin
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.63 30
-
assay at Linum usitatissimum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.63 8.6
-
-
Linum usitatissimum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.63 7.2 9.3 approx. half-maximal activity at pH 7.2 and 9.3, due to substrate stability, Tris buffer is preferred over glycine-NaOH buffer Linum usitatissimum