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Literature summary extracted from

  • Senn, H.J.; Wagner, M.; Decker, K.
    Ganglioside biosynthesis in rat liver. Characterization of UDPgalactose--glucosylceramide galactosyltransferase and UDPgalactose-GM2 galactosyltransferase (1983), Eur. J. Biochem., 135, 231-236.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.62 Detergents requirement, solubilized enzyme, e.g. Cutscum, Lubrol PX, Triton CF-54, octylglucoside Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.62 Brij 56
-
 Rattus norvegicus
2.4.1.62 cardiolipin
-
 Rattus norvegicus
2.4.1.62 EDTA
-
 Rattus norvegicus
2.4.1.62 SDS
-
 Rattus norvegicus
2.4.1.62 Tween 80
-
 Rattus norvegicus
2.4.1.62 UDP-galactose above 0.2 mM Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.62 0.01
-
 N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-1,4-beta-D-glucosyl-N-acylsphingosine
-
 Rattus norvegicus
2.4.1.62 0.14
-
 UDP-galactose
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.62 Golgi apparatus
-
 Rattus norvegicus 5794
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.62 Mn2+ Co2+ or Mg2+ can substitute to some extent Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.62 UDPgalactose + N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-1,4-beta-D-glucosyl-N-acylsphingosine Rattus norvegicus involved in biosynthesis of gangliosides UDP + D-galactosyl-1,3-beta-N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-D-glucosyl-N-acylsphingosine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.62 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.62 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.62 UDP-galactose + N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-1,4-beta-D-glucosyl-N-acylsphingosine
-
 Rattus norvegicus UDP + D-galactosyl-1,3-beta-N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-D-glucosyl-N-acylsphingosine i.e. GM1 ganglioside or monosialoganglioside ?
2.4.1.62 UDPgalactose + N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-1,4-beta-D-glucosyl-N-acylsphingosine involved in biosynthesis of gangliosides Rattus norvegicus UDP + D-galactosyl-1,3-beta-N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-D-glucosyl-N-acylsphingosine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.62 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.62 6.6 7
-
 Rattus norvegicus