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Literature summary extracted from

  • Forsee, W.T.; Elbein, A.D.
    Biosynthesis of mannosyl- and glucosyl-phosphoryl-polyprenols in cotton fibers (1973), J. Biol. Chem., 248, 2858-2867.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.54 Triton X-100 at high concentrations Gossypium hirsutum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.54 0.00067
-
 GDPmannose
-
 Gossypium hirsutum
2.4.1.54 0.02 0.04 ficaprenyl phosphate
-
 Gossypium hirsutum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.54 membrane membrane-bound Mycolicibacterium smegmatis 16020
-
2.4.1.54 membrane membrane-bound Gossypium hirsutum 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.54 Mg2+ requirement Mycolicibacterium smegmatis
2.4.1.54 Mg2+ requirement Gossypium hirsutum
2.4.1.54 Mg2+ optimal concentration: 15 mM, facilitates interaction of lipid and enzyme Mycolicibacterium smegmatis
2.4.1.54 Mg2+ optimal concentration: 15 mM, facilitates interaction of lipid and enzyme Gossypium hirsutum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.54 GDPmannose + phospholipid Gossypium hirsutum polyprenol type, involved in glycophospholipid biosynthesis GDP + mannosyl-1-phospholipid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.54 Gossypium hirsutum
-
 12-15 days after anthesis
-
2.4.1.54 Mycolicibacterium smegmatis
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.54 fiber
-
 Gossypium hirsutum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.54 GDPmannose + ficaprenyl phosphate effective substrate Gossypium hirsutum GDP + mannosyl-1-phosphorylficaprenol
-
 ?
2.4.1.54 GDPmannose + phospholipid polyprenol type, involved in glycophospholipid biosynthesis Gossypium hirsutum GDP + mannosyl-1-phospholipid
-
 ?
2.4.1.54 GDPmannose + polyisoprenyl phosphate endogenous lipid moiety of the glycolipid is a polyprenol and similar to undecaprenyl phosphate, but probably not identical with it, reaction is reversed by GDP, but not by GMP Gossypium hirsutum GDP + mannosyl-phosphorylpolyisoprenol
-
 r
2.4.1.54 GDPmannose + undecaprenyl phosphate reaction is reversed by GDP Mycolicibacterium smegmatis GDP + D-mannosyl-1-phosphoundecaprenol
-
 r
2.4.1.54 additional information no substrates: phosphatidylglycerol, phosphatidylethanolamine, phosphatidylserine, Triton X-100 Gossypium hirsutum ?
-
 ?
2.4.1.54 UDPglucose + ficaprenyl phosphate in the reverse reaction UMP is no substrate Gossypium hirsutum GDP + glucosyl-1-phosphorylficaprenol
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.54 additional information
-
 assay at room temperature Gossypium hirsutum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.54 7.5
-
 Tris-maleate buffer Gossypium hirsutum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.54 6.2 9 Tris buffer, about half-maximal activity at pH 6.2 and 63% of maximal activity at pH 9.0 Gossypium hirsutum