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Literature summary extracted from

  • Chin, T.; Burger, M.M.; Glaser, L.
    Synthesis of teichoic acids. VI. The formation of multiple wall polymers in Bacillus subtilis W-23 (1966), Arch. Biochem. Biophys., 116, 358-367.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.53 0.001
-
 poly(ribitol phosphate) molar concentration of the polymer Bacillus subtilis
2.4.1.53 0.017 0.08 poly(ribitol phosphate) concentration as ribitol units Bacillus subtilis
2.4.1.53 0.12
-
 UDP-D-glucose
-
 Bacillus subtilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.53 cell wall bound, enzyme content is constant at all stages of growth Bacillus subtilis 5618
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.53 CaCl2 absolute requirement for the addition of a divalent cation, optimal concentration: 0.01 M Bacillus subtilis
2.4.1.53 MgCl2 absolute requirement for the addition of a divalent cation, optimal concentration: 0.05 M Bacillus subtilis
2.4.1.53 MnCl2 absolute requirement for the addition of a divalent cation, optimal concentration 0.01 M Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.53 UDP-D-glucose + poly(ribitol phosphate) Bacillus subtilis biosynthesis of teichoic acids UDP + glucosyl-poly(ribitol phosphate)
-
 ?
2.4.1.53 UDP-D-glucose + poly(ribitol phosphate) Bacillus subtilis W-23 biosynthesis of teichoic acids UDP + glucosyl-poly(ribitol phosphate)
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.53 Bacillus subtilis
-
 W-23
-
2.4.1.53 Bacillus subtilis W-23
-
 W-23
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.53 W-23, partial Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.53 additional information
-
-
 Bacillus subtilis

Storage Stability

EC Number Storage Stability Organism
2.4.1.53 -20°C, crude preparation, stable for several months Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.53 UDP-D-glucose + poly(ribitol phosphate) polyglycerol phosphate is no substrate Bacillus subtilis UDP + glucosyl-poly(ribitol phosphate)
-
 ?
2.4.1.53 UDP-D-glucose + poly(ribitol phosphate) biosynthesis of teichoic acids Bacillus subtilis UDP + glucosyl-poly(ribitol phosphate)
-
 ?
2.4.1.53 UDP-D-glucose + poly(ribitol phosphate) polyglycerol phosphate is no substrate Bacillus subtilis W-23 UDP + glucosyl-poly(ribitol phosphate)
-
 ?
2.4.1.53 UDP-D-glucose + poly(ribitol phosphate) biosynthesis of teichoic acids Bacillus subtilis W-23 UDP + glucosyl-poly(ribitol phosphate)
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.53 37
-
 assay at Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.53 7
-
-
 Bacillus subtilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.53 6 8 62% of maximal activity at pH 6.0, 70% of maximal activity at pH 8.0 Bacillus subtilis