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Literature summary extracted from

  • Risteli, L.; Myllylä, R.; Kivirikko, K.I.
    Affinity chromatography of collagen glycosyltransferases on collagen linked to agarose (1976), Eur. J. Biochem., 67, 197-202.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.4.1.50 gel filtration inactivates Gallus gallus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.50 Mn2+
-
 Gallus gallus
2.4.1.66 Mn2+ requirement Gallus gallus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.66 50000 55000 gel filtration Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.50 Gallus gallus
-
 white Leghorn chicken
-
2.4.1.66 Gallus gallus
-
 white Leghorn chicken
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.50 1000fold Gallus gallus
2.4.1.50 partial Gallus gallus
2.4.1.66 5000fold Gallus gallus
2.4.1.66 affinity chromatography Gallus gallus
2.4.1.66 partial Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.50 embryo 14-15 days old Gallus gallus
-
2.4.1.66 embryo 14-15 days old Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.50 0.0147
-
-
 Gallus gallus
2.4.1.66 0.065 0.082
-
 Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.50 UDPgalactose + procollagen 5-hydroxy-L-lysine denatured citrat-soluble rat skin collagen Gallus gallus UDP + procollagen 5-(D-galactosyloxy)-L-lysine beta-glycosidic bond ?
2.4.1.66 UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen peptides prepared by collagenase-digestion from bovine Achilles tendon collagen Gallus gallus UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen
-
 ?
2.4.1.66 UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen peptides prepared from citrate-soluble rat skin collagen Gallus gallus UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen
-
 ?
2.4.1.66 UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen heat-denatured citrate-soluble rat skin collagen Gallus gallus UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.50 37
-
 assay at Gallus gallus