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Literature summary extracted from

  • Chludzinski, A.M.; Germaine, G.R.; Schachtele, C.F.
    Purification and properties of dextransucrase from Streptococcus mutans (1974), J. Bacteriol., 118, 1-7.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.5 2-mercaptoethanol 120 mM, 17% inhibition Streptococcus mutans
2.4.1.5 Ag+ 1 mM, 94% inhibition Streptococcus mutans
2.4.1.5 D-fructose competitive Streptococcus mutans
2.4.1.5 dithiothreitol 100 mM, 41% inhibition Streptococcus mutans
2.4.1.5 Hg2+ 1 mM, 35% inhibition Streptococcus mutans
2.4.1.5 SDS 3.5 M; complete inhibition Streptococcus mutans
2.4.1.5 Urea 8 M, complete inhibition Streptococcus mutans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.5 3
-
 sucrose
-
 Streptococcus mutans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.5 94000
-
 gel filtration Streptococcus mutans

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.5 Streptococcus mutans
-
 6715
-
2.4.1.5 Streptococcus mutans 6715
-
 6715
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.5 6715 Streptococcus mutans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.5 culture medium
-
 Streptococcus mutans
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.5 additional information
-
-
 Streptococcus mutans

Storage Stability

EC Number Storage Stability Organism
2.4.1.5 4°C or -70°C, stable during extended storage Streptococcus mutans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.5 sucrose + (1,6-alpha-D-glucosyl)n
-
 Streptococcus mutans D-fructose + (1,6-alpha-D-glucosyl)n+1
-
 ?
2.4.1.5 sucrose + (1,6-alpha-D-glucosyl)n
-
 Streptococcus mutans 6715 D-fructose + (1,6-alpha-D-glucosyl)n+1
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.5 34 42
-
 Streptococcus mutans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.5 45
-
 20 min, substantial loss of activity above Streptococcus mutans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.5 5.5
-
-
 Streptococcus mutans

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.5 27
-
 fructose
-
 Streptococcus mutans