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Literature summary extracted from

  • Kobayashi, M.; Matsuda, K.
    Purification and properties of the extracellular dextransucrase from Leuconostoc mesenteroides NRRL B-1299 (1976), J. Biochem., 79, 1301-1308.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.5 Dextrans activity of enzyme N is more effectively stimulated than enzyme I Leuconostoc mesenteroides

General Stability

EC Number General Stability Organism
2.4.1.5 maximal stabilization of soluble enzyme by 5 mM Ca2+ Leuconostoc mesenteroides

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.5 Ca2+ 1 mM, stimulates activity of enzyme N, enzyme I is inhibited Leuconostoc mesenteroides
2.4.1.5 Co2+ 1 mM, stimulates activity of enzyme N, enzyme I is inhibited Leuconostoc mesenteroides
2.4.1.5 EDTA enzyme I not affected, enzyme N inhibited, inhibition overcome by Ca2+ Leuconostoc mesenteroides
2.4.1.5 Fe2+ 1 mM, stimulates activity of enzyme N, enzyme I is inhibited Leuconostoc mesenteroides
2.4.1.5 Guanidine-HCl 0.025 M, 65% inhibition of enzyme N, no effect on enzyme I Leuconostoc mesenteroides
2.4.1.5 Mg2+ 1 mM, stimulates activity of enzyme N, enzyme I is inhibited Leuconostoc mesenteroides
2.4.1.5 Mn2+ enzyme I and N, strong inhibition Leuconostoc mesenteroides
2.4.1.5 phenylmercuric acetate
-
 Leuconostoc mesenteroides
2.4.1.5 SDS
-
 Leuconostoc mesenteroides
2.4.1.5 Urea 0.25 M, enzyme N completely inactivated, enzyme I retains 60% of its activity Leuconostoc mesenteroides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.5 additional information
-
 additional information effect of temperature on KM-value Leuconostoc mesenteroides

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.5 extracellular
-
 Leuconostoc mesenteroides
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.5 Ca2+ stimulates activity of enzyme N Leuconostoc mesenteroides
2.4.1.5 Co2+ 1 mM, stimulates activity of enzyme N, enzyme I is inhibited Leuconostoc mesenteroides
2.4.1.5 Fe2+ 1 mM, stimulates activity of enzyme N Leuconostoc mesenteroides
2.4.1.5 Mg2+ 1 mM, stimulates activity of enzyme N Leuconostoc mesenteroides
2.4.1.5 additional information 2 enzyme forms: I and N, enzyme I is gradually converted into enzyme N upon ageing, conversion is stimulated in the presence of NaCl Leuconostoc mesenteroides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.5 48000
-
 x * 48000, enzyme I, SDS-PAGE Leuconostoc mesenteroides

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.5 Leuconostoc mesenteroides
-
 NRRL B-1299
-
2.4.1.5 Leuconostoc mesenteroides NRRL B-1299
-
 NRRL B-1299
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.5 2 forms: I and N, enzyme I is gradually converted into enzyme N upon ageing, conversion is stimulated in the presence of NaCl Leuconostoc mesenteroides
2.4.1.5 NRRL B-1299 Leuconostoc mesenteroides

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.5 culture medium
-
 Leuconostoc mesenteroides
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.5 additional information
-
-
 Leuconostoc mesenteroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.5 sucrose + (1,6-alpha-D-glucosyl)n
-
 Leuconostoc mesenteroides D-fructose + (1,6-alpha-D-glucosyl)n+1
-
 ?
2.4.1.5 sucrose + (1,6-alpha-D-glucosyl)n
-
 Leuconostoc mesenteroides NRRL B-1299 D-fructose + (1,6-alpha-D-glucosyl)n+1
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.5 ? x * 48000, enzyme I, SDS-PAGE Leuconostoc mesenteroides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.5 35
-
 enyme N Leuconostoc mesenteroides
2.4.1.5 40 45 enzyme I Leuconostoc mesenteroides

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.5 35
-
 10 min, enzyme I and N stable Leuconostoc mesenteroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.5 5.5
-
 enzyme N Leuconostoc mesenteroides
2.4.1.5 6
-
 enzyme I Leuconostoc mesenteroides

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.5 5.5
-
 4°C, 24 h, enzyme N is stable Leuconostoc mesenteroides
2.4.1.5 6
-
 4°C, 24 h, enzyme I is stable Leuconostoc mesenteroides