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Literature summary extracted from

  • Miller, A.W.; Eklund, S.H.; Robyt, J.F.
    Milligram to gram scale purification and characterization of dextransucrase from Leuconostoc mesenteroides NRRL B-512F (1986), Carbohydr. Res., 147, 119-133.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.5 12 16 sucrose
-
 Leuconostoc mesenteroides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.5 additional information
-
 the enzyme has two major forms: MW 177000 Da and MW 158000 Da Leuconostoc mesenteroides

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.5 Leuconostoc mesenteroides
-
 NRRl B-512(F)
-
2.4.1.5 Leuconostoc mesenteroides NRRl B-512(F)
-
 NRRl B-512(F)
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.5 2 forms: MW 177000 and MW 158000 Leuconostoc mesenteroides
2.4.1.5 NRRL B-512(F) Leuconostoc mesenteroides

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.5 90 170
-
 Leuconostoc mesenteroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.5 sucrose + (1,6-alpha-D-glucosyl)n
-
 Leuconostoc mesenteroides D-fructose + (1,6-alpha-D-glucosyl)n+1
-
 ?
2.4.1.5 sucrose + (1,6-alpha-D-glucosyl)n
-
 Leuconostoc mesenteroides NRRl B-512(F) D-fructose + (1,6-alpha-D-glucosyl)n+1
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.5 5 5.5
-
 Leuconostoc mesenteroides