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Literature summary extracted from

  • Sheth, K.; Alexander, J.K.
    Purification and properties of beta-1,4-oligoglucan:orthophosphate glucosyltransferase from Clostridium thermocellum (1969), J. Biol. Chem., 244, 457-464.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.49 additional information not affected by pyridoxal 5'-phosphate, adenine nucleotides, sugar phosphates and D-glucose Acetivibrio thermocellus
2.4.1.49 Reducing agents e.g. cysteine, dithiothreitol, reduced glutathione, absolute requirement Acetivibrio thermocellus

General Stability

EC Number General Stability Organism
2.4.1.49 absolute requirement for a reducing agent Acetivibrio thermocellus
2.4.1.49 cysteine, 50 mM, stabilizes Acetivibrio thermocellus
2.4.1.49 DTT, 40 mM, stabilizes Acetivibrio thermocellus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.49 additional information not affected by pyridoxal 5'-phosphate, adenine nucleotides, sugar phosphates and D-glucose Acetivibrio thermocellus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.49 0.13
-
phosphate with cellotriose Acetivibrio thermocellus
2.4.1.49 0.19
-
phosphate with cellotetraose Acetivibrio thermocellus
2.4.1.49 0.24
-
phosphate with cellopentaose Acetivibrio thermocellus
2.4.1.49 0.26
-
phosphate with cellohexaose Acetivibrio thermocellus
2.4.1.49 0.37
-
cellohexaose
-
Acetivibrio thermocellus
2.4.1.49 1
-
cellotriose
-
Acetivibrio thermocellus
2.4.1.49 1
-
cellotetraose
-
Acetivibrio thermocellus
2.4.1.49 1
-
cellopentaose
-
Acetivibrio thermocellus
2.4.1.49 1.2
-
cellobiose with glucose 1-phosphate Acetivibrio thermocellus
2.4.1.49 4.7
-
glucose 1-phosphate with cellobiose Acetivibrio thermocellus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.49 additional information no activation with 1 mM and 10 mM concentration of Mg2+, Mn2+, Ca2+, Zn2+, Sn2+, Al3+, Cu2+, Ni2+, Co2+, Fe2+ Acetivibrio thermocellus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.49 (1,4-beta-D-glucosyl)n + phosphate Acetivibrio thermocellus involved in the catabolism of cellulose (1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
-
r
2.4.1.49 (1,4-beta-D-glucosyl)n + phosphate Acetivibrio thermocellus 651 involved in the catabolism of cellulose (1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
-
r

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.49 Acetivibrio thermocellus
-
-
-
2.4.1.49 Acetivibrio thermocellus 651
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.4.1.49 O2-sensitive, enzyme is inactive in the absence of cysteine or DTT Acetivibrio thermocellus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.49 partial Acetivibrio thermocellus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.49 11.33
-
partially purified enzyme Acetivibrio thermocellus

Storage Stability

EC Number Storage Stability Organism
2.4.1.49 -5°C, at least 4 months Acetivibrio thermocellus
2.4.1.49 -5°C, crude enzyme preparation, at least 6 months Acetivibrio thermocellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.49 (1,4-beta-D-glucosyl)n + phosphate
-
Acetivibrio thermocellus (1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
-
r
2.4.1.49 (1,4-beta-D-glucosyl)n + phosphate involved in the catabolism of cellulose Acetivibrio thermocellus (1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
-
r
2.4.1.49 (1,4-beta-D-glucosyl)n + phosphate
-
Acetivibrio thermocellus 651 (1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
-
r
2.4.1.49 (1,4-beta-D-glucosyl)n + phosphate involved in the catabolism of cellulose Acetivibrio thermocellus 651 (1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
-
r
2.4.1.49 additional information no phosphorolysis of cellobiose, cellulose, laminaritriose, melezitose or raffinose. Overview of cellobiose-analogues as glycosyl acceptors Acetivibrio thermocellus ?
-
?
2.4.1.49 additional information no phosphorolysis of cellobiose, cellulose, laminaritriose, melezitose or raffinose. Overview of cellobiose-analogues as glycosyl acceptors Acetivibrio thermocellus 651 ?
-
?
2.4.1.49 phosphate + cellohexaose
-
Acetivibrio thermocellus cellopentaose + glucose 1-phosphate
-
r
2.4.1.49 phosphate + cellopentaose
-
Acetivibrio thermocellus cellotetraose + glucose 1-phosphate
-
r
2.4.1.49 phosphate + cellotetraose
-
Acetivibrio thermocellus cellotriose + glucose 1-phosphate
-
r
2.4.1.49 phosphate + cellotetraose
-
Acetivibrio thermocellus 651 cellotriose + glucose 1-phosphate
-
r
2.4.1.49 phosphate + cellotriose
-
Acetivibrio thermocellus cellobiose + glucose 1-phosphate
-
r
2.4.1.49 phosphate + cellotriose
-
Acetivibrio thermocellus 651 cellobiose + glucose 1-phosphate
-
r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.49 37
-
assay at Acetivibrio thermocellus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.49 7.5
-
-
Acetivibrio thermocellus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.49 5.5 9 10% of maximal activity at pH 5.5, 15% of maximal activity at pH 9.0, Tris-acetate buffer Acetivibrio thermocellus

Cofactor

EC Number Cofactor Comment Organism Structure
2.4.1.49 additional information contains no pyridoxyl 5'-phosphate Acetivibrio thermocellus