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Literature summary extracted from

  • Ankel, H.; Ankel, E.; Schutzbach, J.S.
    Mannosyl transfer in Cryptococcus laurentii (1970), J. Biol. Chem., 245, 3945-3955.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.4.1.48 repeated freezing and thawing reduces activity Cryptococcus laurentii var. flavescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.48 0.14
-
 GDP-mannose
-
 Cryptococcus laurentii var. flavescens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.48 membrane cell wall associated Cryptococcus laurentii var. flavescens 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.48 Ca2+ 20 mM, required, 15% of activity compared to Mn2+ Cryptococcus laurentii var. flavescens
2.4.1.48 Co2+ 20 mM, required, 16% of activity compared to Mn2+ Cryptococcus laurentii var. flavescens
2.4.1.48 Cu2+ 20 mM, required, about 1% of activity compared to Mn2+ Cryptococcus laurentii var. flavescens
2.4.1.48 Mg2+ 20 mM, required, 35% of activity compared to Mn2+ Cryptococcus laurentii var. flavescens
2.4.1.48 Mn2+ 20 mM, required, 100% activity Cryptococcus laurentii var. flavescens
2.4.1.48 additional information absolute requirement for a divalent cation Cryptococcus laurentii var. flavescens
2.4.1.48 Ni2+ 20 mM, required, 4% of activity compared to Mn2+ Cryptococcus laurentii var. flavescens
2.4.1.48 Zn2+ 20 mM, required, 16% of activity compared to Mn2+ Cryptococcus laurentii var. flavescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.48 GDP-mannose + heteroglycan Cryptococcus laurentii var. flavescens
-
 GDP + 1,2(or 1,3)-alpha-D-mannosylheteroglycan
-
 ?
2.4.1.48 GDP-mannose + heteroglycan Cryptococcus laurentii var. flavescens NRRL Y-1401
-
 GDP + 1,2(or 1,3)-alpha-D-mannosylheteroglycan
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.48 Cryptococcus laurentii var. flavescens
-
 NRRL Y-1401
-
2.4.1.48 Cryptococcus laurentii var. flavescens NRRL Y-1401
-
 NRRL Y-1401
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.48 partial Cryptococcus laurentii var. flavescens

Storage Stability

EC Number Storage Stability Organism
2.4.1.48 -20°C, stable for at least 4 weeks Cryptococcus laurentii var. flavescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.48 GDP-mannose + heteroglycan
-
 Cryptococcus laurentii var. flavescens GDP + 1,2(or 1,3)-alpha-D-mannosylheteroglycan
-
 ?
2.4.1.48 GDP-mannose + heteroglycan
-
 Cryptococcus laurentii var. flavescens NRRL Y-1401 GDP + 1,2(or 1,3)-alpha-D-mannosylheteroglycan
-
 ?
2.4.1.48 GDP-mannose + heteropolysaccharide heteropolysaccharide containing mannosyl, galactosyl, xylosyl units Cryptococcus laurentii var. flavescens GDP + 1,2(or 1,3)-alpha-D-mannosylheteropolysaccharide
-
 ?
2.4.1.48 GDP-mannose + heteropolysaccharide heteropolysaccharide containing mannosyl, galactosyl, xylosyl units Cryptococcus laurentii var. flavescens NRRL Y-1401 GDP + 1,2(or 1,3)-alpha-D-mannosylheteropolysaccharide
-
 ?
2.4.1.48 additional information mannose-1-phosphate and mannose are no substrates Cryptococcus laurentii var. flavescens ?
-
 ?
2.4.1.48 additional information mannose-1-phosphate and mannose are no substrates Cryptococcus laurentii var. flavescens NRRL Y-1401 ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.48 25
-
 assay at Cryptococcus laurentii var. flavescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.48 7.5
-
-
 Cryptococcus laurentii var. flavescens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.48 6 10 20% of maximal activity at pH 6 and pH 10 Cryptococcus laurentii var. flavescens