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Literature summary extracted from

  • Marechal, E.; Awai, K.; Block, M.A.; Brun, D.; Masuda, T.; Shimada, H.; Takamiya, K.I.; Ohta, H.; Joyard, J.
    The multigenic family of monogalactosyl diacylglycerol synthases (2000), Biochem. Soc. Trans., 28, 732-738.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.46 expression in Escherichia coli Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.46 chloroplast envelope
-
 Spinacia oleracea 9941
-
2.4.1.46 chloroplast envelope
-
 Pisum sativum 9941
-
2.4.1.46 chloroplast envelope
-
 Cucumis sativus 9941
-
2.4.1.46 chloroplast envelope
-
 Arabidopsis thaliana 9941
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.46 46870
-
 x * 46870, mature isoenzyme mgd A, deduced from nucleotide sequence Arabidopsis thaliana
2.4.1.46 52730
-
 x * 52730, mature isoenzyme mgd B, deduced from nucleotide sequence Arabidopsis thaliana
2.4.1.46 52860
-
 x * 52860, mature isoenzyme mgd C, deduced from nucleotide sequence Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.46 UDPgalactose + 1,2-diacylglycerol Spinacia oleracea last step in monogalactosyldiacylglycerol biosynthesis UDP + 3-beta-D-galactosyl-1,2-diacylglycerol
-
 ?
2.4.1.46 UDPgalactose + 1,2-diacylglycerol Pisum sativum last step in monogalactosyldiacylglycerol biosynthesis UDP + 3-beta-D-galactosyl-1,2-diacylglycerol
-
 ?
2.4.1.46 UDPgalactose + 1,2-diacylglycerol Cucumis sativus last step in monogalactosyldiacylglycerol biosynthesis UDP + 3-beta-D-galactosyl-1,2-diacylglycerol
-
 ?
2.4.1.46 UDPgalactose + 1,2-diacylglycerol Arabidopsis thaliana last step in monogalactosyldiacylglycerol biosynthesis UDP + 3-beta-D-galactosyl-1,2-diacylglycerol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.46 Arabidopsis thaliana O81770 isoenzyme MGD A
-
2.4.1.46 Arabidopsis thaliana O82730 isoenzyme mgd B
-
2.4.1.46 Arabidopsis thaliana Q9SI93 isoenzyme mgd C
-
2.4.1.46 Cucumis sativus
-
-
-
2.4.1.46 Pisum sativum
-
-
-
2.4.1.46 Spinacia oleracea
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.46 leaf
-
 Spinacia oleracea
-
2.4.1.46 leaf
-
 Pisum sativum
-
2.4.1.46 leaf
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.46 UDP-galactose + 1,2-diacylglycerol
-
 Spinacia oleracea UDP + 3-beta-D-galactosyl-1,2-diacylglycerol
-
 ?
2.4.1.46 UDP-galactose + 1,2-diacylglycerol
-
 Pisum sativum UDP + 3-beta-D-galactosyl-1,2-diacylglycerol
-
 ?
2.4.1.46 UDP-galactose + 1,2-diacylglycerol
-
 Cucumis sativus UDP + 3-beta-D-galactosyl-1,2-diacylglycerol
-
 ?
2.4.1.46 UDP-galactose + 1,2-diacylglycerol
-
 Arabidopsis thaliana UDP + 3-beta-D-galactosyl-1,2-diacylglycerol
-
 ?
2.4.1.46 UDP-galactose + 1,2-dilinoleoylglycerol
-
 Spinacia oleracea UDP + 3-beta-D-galactosyl-1,2-dilinoleoylglycerol
-
 ?
2.4.1.46 UDP-galactose + 1,2-dilinoleoylglycerol
-
 Arabidopsis thaliana UDP + 3-beta-D-galactosyl-1,2-dilinoleoylglycerol
-
 ?
2.4.1.46 UDP-galactose + 1,2-dilinoleoylglycerol preferred substrate Arabidopsis thaliana UDP + 3-beta-D-galactosyl-1,2-dilinoleoylglycerol
-
 ?
2.4.1.46 UDP-galactose + 1,2-dioleoylglycerol
-
 Spinacia oleracea UDP + 3-beta-D-galactosyl-1,2-dioleoylglycerol
-
 ?
2.4.1.46 UDP-galactose + 1,2-dioleoylglycerol
-
 Arabidopsis thaliana UDP + 3-beta-D-galactosyl-1,2-dioleoylglycerol
-
 ?
2.4.1.46 UDPgalactose + 1,2-diacylglycerol last step in monogalactosyldiacylglycerol biosynthesis Spinacia oleracea UDP + 3-beta-D-galactosyl-1,2-diacylglycerol
-
 ?
2.4.1.46 UDPgalactose + 1,2-diacylglycerol last step in monogalactosyldiacylglycerol biosynthesis Pisum sativum UDP + 3-beta-D-galactosyl-1,2-diacylglycerol
-
 ?
2.4.1.46 UDPgalactose + 1,2-diacylglycerol last step in monogalactosyldiacylglycerol biosynthesis Cucumis sativus UDP + 3-beta-D-galactosyl-1,2-diacylglycerol
-
 ?
2.4.1.46 UDPgalactose + 1,2-diacylglycerol last step in monogalactosyldiacylglycerol biosynthesis Arabidopsis thaliana UDP + 3-beta-D-galactosyl-1,2-diacylglycerol
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.46 ? x * 52730, mature isoenzyme mgd B, deduced from nucleotide sequence Arabidopsis thaliana
2.4.1.46 ? x * 46870, mature isoenzyme mgd A, deduced from nucleotide sequence Arabidopsis thaliana
2.4.1.46 ? x * 52860, mature isoenzyme mgd C, deduced from nucleotide sequence Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.46 6.3
-
 isoenzyme mgd A Arabidopsis thaliana
2.4.1.46 7.5
-
 isoenzyme mgd A Arabidopsis thaliana
2.4.1.46 8.5
-
 isoenzyme mgd C Arabidopsis thaliana