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Literature summary extracted from

  • Miege, C.; Marechal, E.; Shimojima, M.; Awai, K.; Block, M.A.; Ohta, H.; Takamiya, K.I.; Douce, R.; Joyard, J.
    Biochemical and topological properties of type A MGDG synthase, a spinach chloroplast envelope enzyme catalyzing the synthesis of both prokaryotic and eukaryotic MGDG (1999), Eur. J. Biochem., 265, 990-1001.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.46 expression of isoenzyme MGD A in Escherichia coli Spinacia oleracea

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.46 N-ethylmaleimide 0.15 mM, 65% inactivation after 10 min, recombinant isoenzyme MGD A, preincubation with diacylglycerol and/or phosphatidylglycerol protects Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.46 chloroplast envelope inner membrane Spinacia oleracea 9941
-
2.4.1.46 chloroplast envelope monotopic membrane enzyme imbedded within 1 of the 2 leaflets Spinacia oleracea 9941
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.46 45000
-
 x * 45000, most likely a dimer, processed mature enzyme, immunoblot Spinacia oleracea
2.4.1.46 58000
-
 x * 58000, precursor, deduced from nucleotide sequence Spinacia oleracea
2.4.1.46 97000
-
 inactivation after gamma-ray irradiation Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.46 Spinacia oleracea Q9SM44 isoenzyme MGD A
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.46 leaf
-
 Spinacia oleracea
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.46 UDP-galactose + 1,2-diacylglycerol
-
 Spinacia oleracea UDP + 3-beta-D-galactosyl-1,2-diacylglycerol
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.46 ? x * 45000, most likely a dimer, processed mature enzyme, immunoblot Spinacia oleracea
2.4.1.46 ? x * 58000, precursor, deduced from nucleotide sequence Spinacia oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.46 7.5 8.5 recombinant isoenzyme MGD A Spinacia oleracea