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Literature summary extracted from

  • Schulte, S.; Stoffel, W.
    UDP galactose:ceramide galactosyltransferase and glutamate/aspartate transporter. Copurification, separation and characterization of the two glycoproteins (1995), Eur. J. Biochem., 233, 947-953.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.47 cloning of the full-length CGT cDNA, open reading frame of 1623 bp encodes a 541 amino acids core protein Rattus norvegicus
2.4.1.47 time-regulated CGT expression Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.47 microsome
-
 Rattus norvegicus
-
-
2.4.1.47 smooth endoplasmic reticulum smooth endoplasmic reticulum, with C-terminal endoplasmic reticulum membrane retention signal, C-terminal 20 residue transmembrane domain as anchor of enzyme in the membrane of the ER Rattus norvegicus 5790
-
2.4.1.62 microsome
-
 Rattus norvegicus
-
-
2.4.1.62 smooth endoplasmic reticulum
-
 Rattus norvegicus 5790
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.47 59000
-
 x * 64000, glycosylated protein, x * 59000, deglycosylated protein with glycopeptidase F, SDS-PAGE Rattus norvegicus
2.4.1.47 64000
-
 x * 64000, glycosylated protein, x * 59000, deglycosylated protein with glycopeptidase F, SDS-PAGE Rattus norvegicus
2.4.1.62 additional information
-
 N-terminal amino acid sequence Rattus norvegicus
2.4.1.62 64000
-
 x * 64000, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.47 additional information Rattus norvegicus oligodendrocyte-specific enzyme is involved in the biosynthesis of the oligodendrocyte- and myelin-specific cerebrosides, time-regulated CGT expression with a peak around postnatal days 15-25 ?
-
 ?
2.4.1.47 additional information Rattus norvegicus Wistar oligodendrocyte-specific enzyme is involved in the biosynthesis of the oligodendrocyte- and myelin-specific cerebrosides, time-regulated CGT expression with a peak around postnatal days 15-25 ?
-
 ?
2.4.1.62 UDPgalactose + N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-1,4-beta-D-glucosyl-N-acylsphingosine Rattus norvegicus key enzyme in the biosynthesis of the oligodendrocyte- and myelin-specific cerebrosides UDP + D-galactosyl-1,3-beta-N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-D-glucosyl-N-acylsphingosine
-
 ?
2.4.1.62 UDPgalactose + N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-1,4-beta-D-glucosyl-N-acylsphingosine Rattus norvegicus Wistar key enzyme in the biosynthesis of the oligodendrocyte- and myelin-specific cerebrosides UDP + D-galactosyl-1,3-beta-N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-D-glucosyl-N-acylsphingosine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.47 Rattus norvegicus
-
 Wistar rats
-
2.4.1.47 Rattus norvegicus
-
 20 days old
-
2.4.1.62 Rattus norvegicus
-
 Wistar
-
2.4.1.62 Rattus norvegicus Wistar
-
 Wistar
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.47 glycoprotein high mannose glycoprotein Rattus norvegicus
2.4.1.47 glycoprotein 3 putative glycosylation sites at Asn-78, Asn-333 and Asn-442, but only Asn-78 and Asn-333 are glycosylated, deglycosylated 59 kDa core protein is still enzymatically active Rattus norvegicus
2.4.1.62 glycoprotein Asn-linked high-mannose glycoprotein Rattus norvegicus
2.4.1.62 glycoprotein glycosylation at Asn 333 and Asn 78 Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.47 copurified with the L-glutamate/aspartate neurotransmitter transporter GLAST-1 of the central nervous system Rattus norvegicus
2.4.1.62
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.47 brain
-
 Rattus norvegicus
-
2.4.1.47 kidney only weakly expressed in Rattus norvegicus
-
2.4.1.47 oligodendrocyte oligodendrocyte-specific enzyme Rattus norvegicus
-
2.4.1.62 brain
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.47 additional information
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.47 additional information oligodendrocyte-specific enzyme is involved in the biosynthesis of the oligodendrocyte- and myelin-specific cerebrosides, time-regulated CGT expression with a peak around postnatal days 15-25 Rattus norvegicus ?
-
 ?
2.4.1.47 additional information oligodendrocyte-specific enzyme is involved in the biosynthesis of the oligodendrocyte- and myelin-specific cerebrosides, time-regulated CGT expression with a peak around postnatal days 15-25 Rattus norvegicus Wistar ?
-
 ?
2.4.1.47 UDPgalactose + 2-(2-hydroxyacyl)sphingosine
-
 Rattus norvegicus 1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP cerebroside ?
2.4.1.47 UDPgalactose + 2-(2-hydroxyacyl)sphingosine
-
 Rattus norvegicus Wistar 1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP cerebroside ?
2.4.1.62 UDP-galactose + N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-1,4-beta-D-glucosyl-N-acylsphingosine
-
 Rattus norvegicus UDP + D-galactosyl-1,3-beta-N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-D-glucosyl-N-acylsphingosine
-
 ?
2.4.1.62 UDP-galactose + N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-1,4-beta-D-glucosyl-N-acylsphingosine
-
 Rattus norvegicus Wistar UDP + D-galactosyl-1,3-beta-N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-D-glucosyl-N-acylsphingosine
-
 ?
2.4.1.62 UDPgalactose + N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-1,4-beta-D-glucosyl-N-acylsphingosine key enzyme in the biosynthesis of the oligodendrocyte- and myelin-specific cerebrosides Rattus norvegicus UDP + D-galactosyl-1,3-beta-N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-D-glucosyl-N-acylsphingosine
-
 ?
2.4.1.62 UDPgalactose + N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-1,4-beta-D-glucosyl-N-acylsphingosine key enzyme in the biosynthesis of the oligodendrocyte- and myelin-specific cerebrosides Rattus norvegicus Wistar UDP + D-galactosyl-1,3-beta-N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-D-glucosyl-N-acylsphingosine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.47 ? x * 64000, glycosylated protein, x * 59000, deglycosylated protein with glycopeptidase F, SDS-PAGE Rattus norvegicus
2.4.1.62 ? x * 64000, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
2.4.1.62 CGT
-
 Rattus norvegicus