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Literature summary extracted from

  • Constantino-Ceccarini, E.; Cestelli, A.
    A novel assay method for the biosynthesis of galactosyl- and glucosylceramides (1981), Methods Enzymol., 72, 384-391.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.4.1.47 rapidly inactivated by repeated freezing and thawing Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.47 Detergents
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.47 membrane membrane-bound Rattus norvegicus 16020
-
2.4.1.47 microsome
-
 Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.47 Mg2+ stimulates Rattus norvegicus
2.4.1.47 Mg2+ Mg2+ required rather than Mn2+ Rattus norvegicus
2.4.1.47 Mn2+ stimulates Rattus norvegicus
2.4.1.47 Mn2+ Mg2+ required rather than Mn2+ Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.47 Rattus norvegicus
-
 Sprague-Dawley rats, 18-25 days old
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.47 brain
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.47 UDPgalactose + 2-(2-hydroxyacyl)sphingosine
-
 Rattus norvegicus 1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.47 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.47 8.5
-
-
 Rattus norvegicus