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Literature summary extracted from

  • Muller, E.; Hinckley, A.; Rothfield, L.
    Studies of phospholipid-requiring bacterial enzymes. III. Purification and properties of uridine diphosphate glucose:lipopolysaccharide glucosyltransferase I (1972), J. Biol. Chem., 247, 2614-2622.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.44 Phospholipid required for activity Salmonella enterica subsp. enterica serovar Typhimurium
2.4.1.44 Phospholipid phosphatidylethanolamine most effective Salmonella enterica subsp. enterica serovar Typhimurium
2.4.1.58 phosphatidylethanolamine requirement, most effective phospholipid, phosphatidylethanolamine containing unsaturated or cyclopropane acyl groups is more effective than that containing saturated acyl groups Salmonella enterica subsp. enterica serovar Typhimurium
2.4.1.58 Phospholipid requirement Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.58 iodoacetamide 5 mM, weak Salmonella enterica subsp. enterica serovar Typhimurium
2.4.1.58 additional information not inhibited by p-chloromercuribenzoate, no detectable inhibition by non-substrate lipopolysaccharides obtained from other mutant strains of Salmonella typhimurium: G30G, G30A, G30, TV119, LT2 Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.58 0.03
-
 lipopolysaccharide in presence of optimal concentrations of phosphatidylethanolamine Salmonella enterica subsp. enterica serovar Typhimurium
2.4.1.58 0.1
-
 lipopolysaccharide in absence of phosphatidylethanolamine Salmonella enterica subsp. enterica serovar Typhimurium
2.4.1.58 0.33
-
 UDPglucose
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.44 membrane bound Salmonella enterica subsp. enterica serovar Typhimurium 16020
-
2.4.1.58 membrane
-
 Salmonella enterica subsp. enterica serovar Typhimurium 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.58 Ba2+ requires divalent cation, in decreasing order of effectiveness: Mg2+, Ba2+, Ca2+, Co2+, relative activities: Mg2+: 1.0, Ba2+: 0.77, Ca2+: 0.65, Co2+: 0.31 Salmonella enterica subsp. enterica serovar Typhimurium
2.4.1.58 Ca2+ requires divalent cation, in decreasing order of effectiveness: Mg2+, Ba2+, Ca2+, Co2+, relative activities: Mg2+: 1.0, Ba2+: 0.77, Ca2+: 0.65, Co2+: 0.31 Salmonella enterica subsp. enterica serovar Typhimurium
2.4.1.58 Co2+ requires divalent cation, in decreasing order of effectiveness: Mg2+, Ba2+, Ca2+, Co2+, relative activities: Mg2+: 1.0, Ba2+: 0.77, Ca2+: 0.65, Co2+: 0.31 Salmonella enterica subsp. enterica serovar Typhimurium
2.4.1.58 Mg2+ requires divalent cation, in decreasing order of effectiveness: Mg2+, Ba2+, Ca2+, Co2+, relative activities: Mg2+: 1.0, Ba2+: 0.77, Ca2+: 0.65, Co2+: 0.31 Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.58 20000
-
 several peaks suggesting aggregation of enzyme, sucrose density gradient centrifugation Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.44 UDP-galactose + lipopolysaccharide Salmonella enterica subsp. enterica serovar Typhimurium transfers D-galactosyl residues to D-glucose in the partially completed core of lipopolysaccharide UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
-
 ir
2.4.1.44 UDP-galactose + lipopolysaccharide Salmonella enterica subsp. enterica serovar Typhimurium G30A transfers D-galactosyl residues to D-glucose in the partially completed core of lipopolysaccharide UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
-
 ir
2.4.1.58 UDPglucose + lipopolysaccharide Salmonella enterica subsp. enterica serovar Typhimurium addition of glucose during the biosynthesis of the lipopolysaccharide component of the cell envelope UDP + D-glucosyllipopolysaccharide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.44 Salmonella enterica subsp. enterica serovar Typhimurium
-
 LT2
-
2.4.1.58 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
2.4.1.58 Salmonella enterica subsp. enterica serovar Typhimurium G30A
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.58 additional information no detectable phospholipid or lipopolysaccharide in the purified enzyme Salmonella enterica subsp. enterica serovar Typhimurium

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.44 partial Salmonella enterica subsp. enterica serovar Typhimurium
2.4.1.58
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.44 0.00284
-
 purified enzyme Salmonella enterica subsp. enterica serovar Typhimurium
2.4.1.58 additional information
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Storage Stability

EC Number Storage Stability Organism
2.4.1.58 4°C, 20% glycerol, for about 5 days: retains full activity, 10% loss of activity per week thereafter Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.44 UDP-galactose + lipopolysaccharide
-
 Salmonella enterica subsp. enterica serovar Typhimurium UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
-
 ir
2.4.1.44 UDP-galactose + lipopolysaccharide transfers D-galactosyl residues to D-glucose in the partially completed core of lipopolysaccharide Salmonella enterica subsp. enterica serovar Typhimurium UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
-
 ir
2.4.1.44 UDP-galactose + lipopolysaccharide
-
 Salmonella enterica subsp. enterica serovar Typhimurium G30A UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
-
 ir
2.4.1.44 UDP-galactose + lipopolysaccharide transfers D-galactosyl residues to D-glucose in the partially completed core of lipopolysaccharide Salmonella enterica subsp. enterica serovar Typhimurium G30A UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
-
 ir
2.4.1.58 additional information poor sugar donor: UDPgalactose, no sugar donors: TDPglucose, CDPglucose, ADPglucose, GDPmannose, glucose, glucose-1-phosphate, galactose, alpha-methylglucoside, beta-methylglucoside, no sugar acceptors: lipopolysaccharides from Salmonella typhimurium G30A, G30G, G30, TV119, LT2 Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
2.4.1.58 additional information poor sugar donor: UDPgalactose, no sugar donors: TDPglucose, CDPglucose, ADPglucose, GDPmannose, glucose, glucose-1-phosphate, galactose, alpha-methylglucoside, beta-methylglucoside, no sugar acceptors: lipopolysaccharides from Salmonella typhimurium G30A, G30G, G30, TV119, LT2 Salmonella enterica subsp. enterica serovar Typhimurium G30A ?
-
 ?
2.4.1.58 UDPglucose + lipopolysaccharide core region of the lipopolysaccharide Salmonella enterica subsp. enterica serovar Typhimurium UDP + D-glucosyllipopolysaccharide
-
 ?
2.4.1.58 UDPglucose + lipopolysaccharide UDPglucose is the only effective glucosyl donor Salmonella enterica subsp. enterica serovar Typhimurium UDP + D-glucosyllipopolysaccharide
-
 ?
2.4.1.58 UDPglucose + lipopolysaccharide glucose is transferred only to the glucose-deficient lipopolysaccharide obtained from Salmonella typhimurium SL1032, a strain lacking the glucosyl transferase system, transfers glucose to the L-glycero-D-mannoheptosyl residue, maximum incorporation of 0.5 mol glucose per mol of heptose Salmonella enterica subsp. enterica serovar Typhimurium UDP + D-glucosyllipopolysaccharide
-
 ?
2.4.1.58 UDPglucose + lipopolysaccharide addition of glucose during the biosynthesis of the lipopolysaccharide component of the cell envelope Salmonella enterica subsp. enterica serovar Typhimurium UDP + D-glucosyllipopolysaccharide
-
 ?
2.4.1.58 UDPglucose + lipopolysaccharide core region of the lipopolysaccharide Salmonella enterica subsp. enterica serovar Typhimurium G30A UDP + D-glucosyllipopolysaccharide
-
 ?
2.4.1.58 UDPglucose + lipopolysaccharide UDPglucose is the only effective glucosyl donor Salmonella enterica subsp. enterica serovar Typhimurium G30A UDP + D-glucosyllipopolysaccharide
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.58 monomer 1 * 20000, additional faint bands: 44,5, 49 and 72 kDa, probably a single polypeptide chain, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.58 37
-
 assay at Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.58 7.5 8.5 Tris-maleate or Tris-Cl buffers Salmonella enterica subsp. enterica serovar Typhimurium