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Literature summary extracted from

  • Doong, R.L.; Liljebjelke, K.; Fralish, G.; Kumar, A.; Mohnen, D.
    Cell-free synthesis of pectin. Identification and partial characterization of polygalaturonate 4-alpha-galaturonosyltransferase and its products from membrane preparations of tobacco cell-suspension cultures (1995), Plant Physiol., 109, 141-152.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.43 0.0089
-
 UDP-D-galacturonic acid
-
 Nicotiana tabacum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.43 KCl 25 mM, 76% stimulation Nicotiana tabacum
2.4.1.43 MnCl2 maximal activation at 0.25 mM Nicotiana tabacum
2.4.1.43 NaCl 25 mM, 76% stimulation Nicotiana tabacum

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.43 Nicotiana tabacum
-
 L. cv. Samsun
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.43 cell suspension culture established from pith callus tissue Nicotiana tabacum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.43 UDP-D-galacturonic acid + (1,4-alpha-D-galacturonosyl)n
-
 Nicotiana tabacum UDP + (1,4-alpha-D-galacturonosyl)n+1 at least 34-89% alpha-1,4-linked homogalacturonan of approx. 105000 Da ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.43 25 30 second minor optimum at 45 or 50°C Nicotiana tabacum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.43 50
-
 30 min, complete inactivation Nicotiana tabacum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.43 7.8
-
-
 Nicotiana tabacum