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Literature summary extracted from

  • Villemez, C.L.; Swanson, A.L.; Hassid, W.Z.
    Properties of a polygalacturonic acid-synthesizing enzyme system from Phaseolus aureus seedlings (1966), Arch. Biochem. Biophys., 116, 446-452.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.43 bovine serum albumin necessary in homogenization media Vigna radiata var. radiata
2.4.1.43 sucrose 400 mM, 5fold increase of activity Vigna radiata var. radiata

General Stability

EC Number General Stability Organism
2.4.1.43 presence of bovine serum albumin is essential for preparation Vigna radiata var. radiata

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.43 CaCl2 weak Vigna radiata var. radiata
2.4.1.43 CuSO4 strong Vigna radiata var. radiata
2.4.1.43 diphosphate weak Vigna radiata var. radiata
2.4.1.43 HgCl2 strong Vigna radiata var. radiata
2.4.1.43 UDP strong Vigna radiata var. radiata
2.4.1.43 UMP strong Vigna radiata var. radiata
2.4.1.43 UTP strong Vigna radiata var. radiata
2.4.1.43 ZnCl2 weak Vigna radiata var. radiata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.43 0.0017
-
 UDP-D-galacturonic acid
-
 Vigna radiata var. radiata

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.43 CoCl2 slight stimulation Vigna radiata var. radiata
2.4.1.43 KCl slight stimulation Vigna radiata var. radiata
2.4.1.43 MgCl2 slight stimulation Vigna radiata var. radiata
2.4.1.43 MnCl2 stimulation, optimal concentration: 1.7 mM Vigna radiata var. radiata
2.4.1.43 MnSO4 stimulation Vigna radiata var. radiata
2.4.1.43 Na2SO4 slight stimulation Vigna radiata var. radiata
2.4.1.43 NaCl slight stimulation Vigna radiata var. radiata
2.4.1.43 NH4Cl slight stimulation Vigna radiata var. radiata

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.43 Vigna radiata var. radiata
-
 mung beans
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.43 partial Vigna radiata var. radiata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.43 seedling
-
 Vigna radiata var. radiata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.43 additional information
-
 highest specific activity in seedlings germinated for 3 days Vigna radiata var. radiata

Storage Stability

EC Number Storage Stability Organism
2.4.1.43 -18°C, 1,7 mM MnCl2, 11 days, 35% loss of activity Vigna radiata var. radiata
2.4.1.43 -18°C, approx. 3 weeks, complete inactivation Vigna radiata var. radiata
2.4.1.43 -18°C, without MnCl2, 11 days, 60% loss of activity Vigna radiata var. radiata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.43 UDP-D-galacturonic acid + (1,4-alpha-D-galacturonosyl)n
-
 Vigna radiata var. radiata UDP + (1,4-alpha-D-galacturonosyl)n+1
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.43 30
-
-
 Vigna radiata var. radiata

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.43
-
-
 24 h, complete inactivation Vigna radiata var. radiata
2.4.1.43 30
-
 20 min, complete inactivation Vigna radiata var. radiata
2.4.1.43 37
-
 5 min, complete inactivation Vigna radiata var. radiata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.43 6
-
-
 Vigna radiata var. radiata

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.43 5.3 7 33% of maximal activity at pH 5.3, 35% of maximal activity at pH 7.0 Vigna radiata var. radiata