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Literature summary extracted from

  • Kojima, M.; Poulton, J.E.; Thayer, S.S.; Conn, E.E.
    Tissue distributions of dhurrin and of enzymes involved in its metabolism in leaves of Sorghum bicolor (1979), Plant Physiol., 63, 1022-1028.
    View publication on PubMedView publication on EuropePMC

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.85 additional information Sorghum bicolor final enzyme of dhurrin biosynthesis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.85 Sorghum bicolor
-
 cv. Moench var. Redland x Greenleaf
-
4.1.2.11 Sorghum bicolor
-
 i.e. Sorghum vulgare
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.85 leaf blade 68% of enzyme activity in epidermal and 32% in mesophyllic tissue Sorghum bicolor
-
2.4.1.85 additional information no activity in bundle sheath extracts Sorghum bicolor
-
2.4.1.85 seedling 6 days old, light-grown, leaf blades Sorghum bicolor
-
4.1.2.11 leaf
-
 Sorghum bicolor
-
4.1.2.11 mesophyll
-
 Sorghum bicolor
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.85 additional information
-
-
 Sorghum bicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.85 additional information final enzyme of dhurrin biosynthesis Sorghum bicolor ?
-
 ?
2.4.1.85 UDPglucose + (S)-4-hydroxymandelonitrile
-
 Sorghum bicolor UDP + (S)-4-hydroxymandelonitrile beta-D-glucoside identical with dhurrin ?
4.1.2.11 (S)-4-Hydroxymandelonitrile
-
 Sorghum bicolor Cyanide + 4-hydroxybenzaldehyde
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.85 30
-
 assay at Sorghum bicolor