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Literature summary extracted from

  • Ten Hagen, K.G.; Tran, D.T.
    A UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase is essential for viability in Drosophila melanogaster (2002), J. Biol. Chem., 277, 22616-22622.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.41 cloning, sequencing and expression of wild type and SF32 mutant recombinant pgant35A gene in COS7 cells Drosophila melanogaster

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.41 L195stop 3775 mutant, more than half of the putative catalytic region is eliminated, early pupal lethality Drosophila melanogaster
2.4.1.41 Q89stop HG8 mutant, truncated protein within the putative stem region, early pupal lethality Drosophila melanogaster
2.4.1.41 R227W SF32 mutant with dramatically reduced activity, early pupal lethality Drosophila melanogaster

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.41 0.02
-
 UDP-GalNAc
-
 Drosophila melanogaster
2.4.1.41 0.595
-
 EA2 peptide
-
 Drosophila melanogaster

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.41 membrane type II membrane protein containing a 23-amino acid transmembrane domain and an 8-amino acid cytoplasmic tail Drosophila melanogaster 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.41 78000
-
 x * 78000, recombinant wild type and SF32 mutant enzyme, SDS-PAGE Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.41 additional information Drosophila melanogaster involved in development, necessary for viability ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.41 Drosophila melanogaster Q8MVS5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.41 recombinant enzyme, expressed in COS7 cells Drosophila melanogaster

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.41 embryo cloning of the pgant35A gene from embryonic cDNA library Drosophila melanogaster
-
2.4.1.41 additional information expression of enzyme throughout embryonic, larval and pupal stages, in adult males and females, expression increases during larval development and continues through pupal and adult stages Drosophila melanogaster
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.41 additional information 519-amino acid putative catalytic region Drosophila melanogaster ?
-
 ?
2.4.1.41 additional information involved in development, necessary for viability Drosophila melanogaster ?
-
 ?
2.4.1.41 UDP-N-acetyl-alpha-D-galactosamine + EA2 peptide
-
 Drosophila melanogaster ?
-
 ?
2.4.1.41 UDP-N-acetyl-D-galactosamine + GalNAc-glycosylated peptide acceptor: MUC5AC-13 glycopeptide Drosophila melanogaster UDP + N-acetyl-D-galactosaminyl-GalNAc-glucosylated peptide
-
 ?
2.4.1.41 UDP-N-acetyl-D-galactosamine + polypeptide acceptors: substantial activity only with EA2 peptide, less active with IgAh peptide Drosophila melanogaster UDP + N-acetyl-D-galactosaminyl-polypeptide
-
 ?
2.4.1.41 UDP-N-acetyl-D-galactosamine + polypeptide transfer of GalNAc to a serine or threonine residue on the acceptor protein Drosophila melanogaster UDP + N-acetyl-D-galactosaminyl-polypeptide
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.41 ? x * 78000, recombinant wild type and SF32 mutant enzyme, SDS-PAGE Drosophila melanogaster

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.41 37
-
 assay at Drosophila melanogaster