EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.41 | expression of secreted GalNAc-T4 in CHO cells | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.41 | fully GalNAc-substituted MUC1 repeat peptide | inhibits activity of rGalNAc-T4 and -T2 | Homo sapiens | |
2.4.1.41 | additional information | elongation of GalNAc by beta3-galactosylation inhibits rGalNAc-T4 activity completely and rGalNAc-T2 activity with respect to the acceptor site GSTA, not inhibited by GlcNAc | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.4.1.41 | Golgi apparatus | - |
Homo sapiens | 5794 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.41 | UDP-N-acetyl-D-galactosamine + polypeptide | Homo sapiens | post-translational, initial reaction in O-linked oligosaccharide biosynthesis | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.41 | Homo sapiens | - |
recombinant GalNAc-T2 and -T4 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.41 | partial | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.41 | additional information | multiple enzyme isoforms | Homo sapiens | ? | - |
? | |
2.4.1.41 | additional information | all known rGalNAc-Ts are characterized by a putative lectin domain in their C-terminal regions | Homo sapiens | ? | - |
? | |
2.4.1.41 | UDP-N-acetyl-D-galactosamine + GalNAc-glycosylated peptide | rGalNAc-T4 is dependent on the presence of GalNAc on acceptor peptides, activity is triggered by a lectin-like interaction of enzyme with the glycopeptide directed by the putative lectin domain found in the C-terminal region, rGalNAc-T2 activity is enhanced on glycosylated acceptor peptides | Homo sapiens | UDP + N-acetyl-D-galactosaminyl-GalNAc-glucosylated peptide | - |
? | |
2.4.1.41 | UDP-N-acetyl-D-galactosamine + polypeptide | site-specific O-glycosylation by rGalNAc-T2 and -T4 is controlled by the primary sequence context and the position and structure of previously introduced O-glycans, acceptors: synthetic mucin-type peptides and glycopeptides derived from tandem repeat regions of MUC1, MUC2 and MUC4, minimum peptide length is required, which is larger for glycosylated substrates, concerted and sequential action of rGalNAc-T2 and -T4 fully glycosylates MUC1, but only partially MUC2 and MUC4 tandem repeat peptides | Homo sapiens | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
2.4.1.41 | UDP-N-acetyl-D-galactosamine + polypeptide | transfer of GalNAc to a serine or threonine residue on the acceptor protein | Homo sapiens | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
2.4.1.41 | UDP-N-acetyl-D-galactosamine + polypeptide | post-translational, initial reaction in O-linked oligosaccharide biosynthesis | Homo sapiens | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.41 | 37 | - |
assay at | Homo sapiens |