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Literature summary extracted from

  • Mendicino, J.; Sangadala, S.
    Purification and characterization of UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase from swine trachea epithelium (1998), Mol. Cell. Biochem., 185, 135-145.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.41 Mn2+ at higher concentrations, 30 mM: 50% inhibition Sus scrofa
2.4.1.41 additional information not inhibited by UTP Sus scrofa
2.4.1.41 UDP competitive Sus scrofa
2.4.1.41 UMP uncompetitive Sus scrofa
2.4.1.102 Mn2+ concentration above 7.5 mM Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.41 additional information
-
 additional information Km: 0.83 mg/ml: deglycosylated Cowper's gland mucin, 1.12 mg/ml: deglycosylated human trachea mucin, 0.94 mg/ml: deglycosylated swine trachea mucin, values for several tryptic peptides derived from them Sus scrofa
2.4.1.41 0.0063
-
 UDP-GalNAc
-
 Sus scrofa
2.4.1.41 0.0141
-
 deglycosylated swine trachea mucin
-
 Sus scrofa
2.4.1.41 0.0175
-
 deglycosylated human trachea mucin
-
 Sus scrofa
2.4.1.102 additional information
-
 additional information
-
 Sus scrofa
2.4.1.102 0.0063
-
 UDP-N-acetyl-D-glucosamine
-
 Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.41 membrane membrane-bound Sus scrofa 16020
-
2.4.1.41 microsome
-
 Sus scrofa
-
-
2.4.1.102 microsome
-
 Sus scrofa
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.41 Mn2+ requirement Sus scrofa
2.4.1.41 Mn2+ maximal activity at 5-7.5 mM, higher concentrations are inhibitory Sus scrofa
2.4.1.102 Mn2+ required, 5-7.5 mM Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.41 70000
-
 gel filtration Sus scrofa
2.4.1.102 70000
-
 gel filtration Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.41 UDP-N-acetyl-D-galactosamine + polypeptide Sus scrofa primary step in the production of mucin-type O-linked oligosaccharide groups in glycoproteins UDP + N-acetyl-D-galactosaminyl-polypeptide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.41 Sus scrofa
-
-
-
2.4.1.102 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.41 affinity chromatography on deglycosylated Cowper's gland mucin bound to Sepharose, 12500fold Sus scrofa
2.4.1.102
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.41 tracheal epithelium
-
 Sus scrofa
-
2.4.1.102 trachea
-
 Sus scrofa
-
2.4.1.102 tracheal epithelium
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.41 2.12
-
-
 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
2.4.1.41 -80°C, washed mucosal membranes, no loss of activity after 1 year Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.41 additional information not: UDP-glucuronate, UDP-xylose, GDP-mannose, GDP-fucose, intact mucins, desialylated mucins, glycolipid acceptors, including globoside and ceramide hexosides, no transfer of GalNAc to terminal sugar residues in oligosaccharide chains Sus scrofa ?
-
 ?
2.4.1.41 additional information not: UDP-GlcNAc Sus scrofa ?
-
 ?
2.4.1.41 additional information not: UDP-Gal Sus scrofa ?
-
 ?
2.4.1.41 UDP-N-acetyl-alpha-D-galactosamine + deglycosylated human trachea mucin
-
 Sus scrofa ?
-
 ?
2.4.1.41 UDP-N-acetyl-alpha-D-galactosamine + deglycosylated swine trachea mucin
-
 Sus scrofa ?
-
 ?
2.4.1.41 UDP-N-acetyl-D-galactosamine + apomucin deglycosylated Cowper's gland, swine and human trachea mucins Sus scrofa UDP + N-acetyl-D-galactosaminyl-apomucin
-
 ?
2.4.1.41 UDP-N-acetyl-D-galactosamine + polypeptide only UDP-GalNAc serves as sugar donor Sus scrofa UDP + N-acetyl-D-galactosaminyl-polypeptide
-
 ?
2.4.1.41 UDP-N-acetyl-D-galactosamine + polypeptide transfer of GalNAc to a serine or threonine residue on the acceptor protein Sus scrofa UDP + N-acetyl-D-galactosaminyl-polypeptide
-
 ?
2.4.1.41 UDP-N-acetyl-D-galactosamine + polypeptide acceptors: deglycosylated Cowper's gland, swine and human trachea mucins, tryptic peptides prepared from them, best substrates are fragments of incompletely deglycosylated mucin polypeptide chains Sus scrofa UDP + N-acetyl-D-galactosaminyl-polypeptide
-
 ?
2.4.1.41 UDP-N-acetyl-D-galactosamine + polypeptide primary step in the production of mucin-type O-linked oligosaccharide groups in glycoproteins Sus scrofa UDP + N-acetyl-D-galactosaminyl-polypeptide
-
 ?
2.4.1.102 additional information attachment of UDPGlcNAc to serine or threonine residue Sus scrofa ?
-
 ?
2.4.1.102 UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-R highly specific for UDP-N-acetyl-D-glucosamine Sus scrofa UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R
-
 ?
2.4.1.102 UDP-N-acetyl-D-glucosamine + trachea mucin acceptor substrate is deglycosylated Sus scrofa ?
-
 ?
2.4.1.102 UDP-N-acetyl-D-glucosamine + trachea mucin porcine or human trachea mucin Sus scrofa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.41 monomer 1 * 70000, SDS-PAGE Sus scrofa
2.4.1.102 monomer 1 * 70000, SDS-PAGE Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.41 37
-
 assay at Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.41 6.7 7.8 broad pH-optimum between pH 6.7-7.8 with maximum at pH 7.2 Sus scrofa
2.4.1.41 7.2
-
 broad pH-optimum between pH 6.7-7.8 with maximum at pH 7.2 Sus scrofa
2.4.1.102 7.2
-
-
 Sus scrofa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.102 6.7 7.8 broad optimum Sus scrofa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.41 0.019
-
 UDP
-
 Sus scrofa
2.4.1.41 0.041
-
 UMP
-
 Sus scrofa