Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Wang, Y.; Abernethy, J.L.; Eckhardt, A.E.; Hill, R.L.
    Purification and characterization of a UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase specific for glycosylation of threonine residues (1992), J. Biol. Chem., 267, 12709-12716.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.41 DTT activates, especially after storage Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.41 Mn2+ Mn2+ is the best activator, maximal activity at 5 mM, 20 and 100 mM result in 10% and 80% inhibition Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.41 additional information
-
 additional information Km: 1.01 mg/ml: ovine apomucin, 2.54 mg/ml: porcine apomucin Sus scrofa
2.4.1.41 0.006
-
 UDP-GalNAc ovine apomucin Sus scrofa
2.4.1.41 7.2
-
 Val-Lys-Thr-Glu-Ala-Thr-Thr-Phe-Ile
-
 Sus scrofa
2.4.1.41 18.6
-
 Val-Leu-Gly-Thr-Thr-Ala-Val
-
 Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.41 membrane membrane-bound Sus scrofa 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.41 Cd2+ less stimulation than by Mn2+ Sus scrofa
2.4.1.41 Co2+ less stimulation than by Mn2+ Sus scrofa
2.4.1.41 Cu2+ less stimulation than by Mn2+ Sus scrofa
2.4.1.41 Mn2+ metal-free enzyme is inactive, Mn2+ is the best activator, maximal activity at 5 mM, half-maximal activity at 0.4 mM MnCl2, 20 and 100 mM result in 10% and 80% inhibition Sus scrofa
2.4.1.41 additional information no reactivation of metal-free enzyme by Mg2+, Zn2+ and Ca2+ Sus scrofa
2.4.1.41 Ni2+ less stimulation than by Mn2+ Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.41 69000
-
 PAGE Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.41 UDP-N-acetyl-D-galactosamine + polypeptide Sus scrofa first step in biosynthesis of O-linked oligosaccharides in submaxillary gland UDP + N-acetyl-D-galactosaminyl-polypeptide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.41 Sus scrofa
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.41 glycoprotein enzyme contains more than one N-linked oligosaccharide Sus scrofa

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.41 1320fold Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.41 submaxillary gland high enzyme concentration Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.41 additional information
-
-
 Sus scrofa
2.4.1.41 1.65
-
-
 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
2.4.1.41 -20°C, 20 mM imidazole-HCl, pH 7.2, 0.1% Triton X-100, 5 mM MnCl2, 60% w/v glycerol, 1 year, stable Sus scrofa
2.4.1.41 4°C, 20 mM imidazole-HCl, pH 7.2, 0.1% Triton X-100, 5 mM MnCl2, 25% w/v glycerol, 1 month, 10% loss of activity Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.41 additional information not: UDP-GlcNAc Sus scrofa ?
-
 ?
2.4.1.41 additional information not: UDP-Gal Sus scrofa ?
-
 ?
2.4.1.41 UDP-N-acetyl-alpha-D-galactosamine + Val-Leu-Gly-Thr-Thr-Ala-Val
-
 Sus scrofa ?
-
 ?
2.4.1.41 UDP-N-acetyl-alpha-D-galactosamine + Val-Lys-Thr-Glu-Ala-Thr-Thr-Phe-Ile
-
 Sus scrofa ?
-
 ?
2.4.1.41 UDP-N-acetyl-D-galactosamine + apomucin porcine apomucin Sus scrofa UDP + N-acetyl-D-galactosaminyl-apomucin
-
 ?
2.4.1.41 UDP-N-acetyl-D-galactosamine + apomucin ovine apomucin Sus scrofa UDP + N-acetyl-D-galactosaminyl-apomucin
-
 ?
2.4.1.41 UDP-N-acetyl-D-galactosamine + polypeptide acceptors: ovine and porcine apomucin, porcine mucin-like synthetic peptides Sus scrofa UDP + N-acetyl-D-galactosaminyl-polypeptide
-
 ?
2.4.1.41 UDP-N-acetyl-D-galactosamine + polypeptide specifically transfers N-acetylgalactosamine from UDP-GalNAc to the hydroxyl group of threonine, no transferase activity towards serine-containing peptides, amino acid sequence adjacent to glycosylated Thr residues may influence the rate of glycosylation Sus scrofa UDP + N-acetyl-D-galactosaminyl-polypeptide
-
 ?
2.4.1.41 UDP-N-acetyl-D-galactosamine + polypeptide first step in biosynthesis of O-linked oligosaccharides in submaxillary gland Sus scrofa UDP + N-acetyl-D-galactosaminyl-polypeptide
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.41 37
-
 assay at Sus scrofa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.41 37
-
 8 h, 20 mM imidazole-HCl, pH 7.2, 0.1% Triton X-100, 5 mM MnCl2, 5% w/v glycerol, 40% loss of activity Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.41 6.8 8.2
-
 Sus scrofa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.41 4.5 9.5 pH 4.5: no activity, pH 6.8: gradual decrease of activity below, pH 9.5: 16% of activity maximum Sus scrofa