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Literature summary extracted from

  • Sugiura, M.; Kawasaki, T.; Yamashina, I.
    Purification and characterization of UDP-GalNAc:polypeptide N-acetylgalactosamine transferase from an ascites hepatoma, AH 66 (1982), J. Biol. Chem., 257, 9501-9507.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.41 EDTA 10 mM, complete inhibition Rattus norvegicus
2.4.1.41 additional information not inhibited by ATP, CDP, GDP Rattus norvegicus
2.4.1.41 UDP competitive Rattus norvegicus
2.4.1.41 UMP uncompetitive Rattus norvegicus
2.4.1.41 UTP competitive Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.41 0.0417
-
 UDP-GalNAc
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.41 membrane membrane-bound Rattus norvegicus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.41 Co2+ less stimulation than by Mn2+ Rattus norvegicus
2.4.1.41 Cu2+ less stimulation than by Mn2+ Rattus norvegicus
2.4.1.41 Mn2+ requirement Rattus norvegicus
2.4.1.41 Mn2+ most effective cation Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.41 54000
-
 gel filtration Rattus norvegicus
2.4.1.41 56000
-
 1 * 56000, SDS-PAGE in presence of 2-mercaptoethanol Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.41 Rattus norvegicus
-
 female albino rats
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.41 48100fold Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.41 hepatoma ascites cell AH-66 ascites hepatoma cells, harvested 6-7 days after the intraperitoneal transfer of ascites fluid from donor rats, activity much higher than in rat liver Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.41 0.39
-
-
 Rattus norvegicus
2.4.1.41 2.12
-
 purified enzyme Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.4.1.41 -20°C, 3 months, stable Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.41 additional information not: lacto-N-fucopentaose I, ceramide dihexosides, ceramide trihexosides, globoside Rattus norvegicus ?
-
 ?
2.4.1.41 UDP-N-acetyl-D-galactosamine + A1 protein
-
 Rattus norvegicus UDP + N-acetyl-D-galactosaminyl-A1 protein
-
 ?
2.4.1.41 UDP-N-acetyl-D-galactosamine + apoantifreeze glycoproteins apoantifreeze glycoprotein of high molecular weight accepts GalNAc much faster than apoantifreeze glycoprotein 8 Rattus norvegicus UDP + N-acetyl-D-galactosaminyl-apoantifreeze glycoproteins
-
 ?
2.4.1.41 UDP-N-acetyl-D-galactosamine + apofetuin lowest rate of transfer Rattus norvegicus UDP + N-acetyl-D-galactosaminyl-apofetuin
-
 ?
2.4.1.41 UDP-N-acetyl-D-galactosamine + apomucin enzymatically prepared apomucin accepts GalNAc much faster than chemically prepared apomucin Rattus norvegicus UDP + N-acetyl-D-galactosaminyl-apomucin
-
 ?
2.4.1.41 UDP-N-acetyl-D-galactosamine + kappa-casein
-
 Rattus norvegicus UDP + N-acetyl-D-galactosaminyl-kappa-casein
-
 ?
2.4.1.41 UDP-N-acetyl-D-galactosamine + polypeptide only UDP-GalNAc serves as sugar donor Rattus norvegicus UDP + N-acetyl-D-galactosaminyl-polypeptide
-
 ?
2.4.1.41 UDP-N-acetyl-D-galactosamine + polypeptide acceptors: apomucin, kappa-casein Rattus norvegicus UDP + N-acetyl-D-galactosaminyl-polypeptide
-
 ?
2.4.1.41 UDP-N-acetyl-D-galactosamine + polypeptide acceptors: A1 protein, apofetuin, apoantifreeze glycoproteins, frequent glycosylation of the abundant serine plus threonine residues of mucins Rattus norvegicus UDP + N-acetyl-D-galactosaminyl-polypeptide
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.41 monomer 1 * 56000, SDS-PAGE in presence of 2-mercaptoethanol Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.41 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.41 7.5
-
-
 Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.41 0.026
-
 UDP
-
 Rattus norvegicus
2.4.1.41 0.0835
-
 UTP
-
 Rattus norvegicus
2.4.1.41 0.347
-
 UMP
-
 Rattus norvegicus